What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
The datasheet that came with my product does not state that material is sterile. Will it contaminate my cells?
Please note that all our products are not sterile unless otherwise indicated on their datasheets. If a product is not…
Tue, Jul 18, 2023
After I’ve made a stock solution, can that solution be thawed and refrozen for multiple uses or should it be aliquotted?
Many small molecules are not easily damaged by freeze/thaw cycles. It is therefore not typically necessary to aliquot stock solutions…
Tue, Jul 18, 2023
The datasheet that came with my product states that solutions need to be made fresh and should not be stored. I do not need to use the entire vial at one, but the amounts needed are to small to weigh out in solid form. How can I handle this?
In many cases such compounds can be dissolved in a volatile solvent, the solution aliquoted in amounts suitable for a…
Tue, Jul 18, 2023
General Information
The solubility of small molecule products, especially those that are hydrophobic can sometimes be a limiting factor in experimental design.…
Tue, Jul 18, 2023
Can samples be frozen before clean up?
No. Samples should be stored at +4 °C after the addition of the stop buffer. They should not be frozen,…
Tue, Jul 18, 2023
Can I use your small molecule compounds as analytical standards and/or calibrants?
All our small molecules are research reagents only. We do not offer chemicals that are certified be used as analytical…
Tue, Jul 18, 2023
Now that i’ve opened my product and used it for the first time, how should i store the leftover material?
Recommendations for storage after initial use may be provided on the product datasheet or certificate of analysis. These vary depending upon…
Tue, Jul 18, 2023
The datasheet that came with my product states that the product needs to be stored under inert gas. What does that mean?
Inert gases such as nitrogen or argon are used to protect compounds that are highly prone to oxidation. To store…
Tue, Jul 18, 2023
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