AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Life Sciences Products
Immunoassay Basics
Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen that…
Tue, Jul 18, 2023
Competitive Immunoassays
In competitive ELISA, the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a reporter…
Tue, Jul 18, 2023
Immunometric Assays
Immunometric assays, also known as sandwich assays, use two antibodies specific to the antigen to capture or “sandwich” antigen in…
Tue, Jul 18, 2023
Edge Effects
Where was the plate incubated? Often times the conditions for ambient incubations can be less than ideal. If there is…
Tue, Jul 18, 2023
Poor Standard Curve
What was used as the standard diluent? Diluents other than the supplied assay buffer may contain interfering substances that can…
Tue, Jul 18, 2023
Poor Precision
Were the wells washed properly? All wells receive the same treatment during the wash step. If some are washed less…
Tue, Jul 18, 2023
Drift
Were reagents brought to room temperature prior to use? If the reagents are not at a constant temperature prior to…
Tue, Jul 18, 2023
Weak Color Development
Was substrate added at the correct point in the assay? See the assay procedure provided in the instruction manual. Were…
Tue, Jul 18, 2023
High Background
How was the plate washed? It is important that the plate is washed thoroughly. If plate washing is troublesome, a…
Tue, Jul 18, 2023
How long can the Cyto-ID® green detection reagent be left incubating with live cells (mins/hours/days)?
We do not recommend to incubate cells with Cyto-ID® green detection reagent for longer that 30 minutes at 37°C. In…
Tue, Jul 18, 2023