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Mito-ID cell staining is too strong
Mitochondrial membrane potential dye is too concentrated for the cell type used. Optimize for different cell types by diluting the…
Tue, Jul 18, 2023
I am having difficulty using my current lot of antibody for my intended application. What should I do?
Please provide as many details about the experiment as possible as well as an example of the data generated. Experiment details…
Tue, Jul 18, 2023
Cells do not exhibit fluorescence after incubation with the detection reagent.
Cell viability is low. Cells should be in log growth phase. Cell samples cannot be kept longer than 6 hours…
Tue, Jul 18, 2023
Fluorescence does not increase after incubation with inhibitor.
Cells do not express any MDR1, MRP1/2 or BCRP. Use a positive control cell type expressing corresponding ABC transporter.
Tue, Jul 18, 2023
There are differences in MFI (mean fluorescence intensity) values between cell lines.
Detection reagent accumulation may be influenced by cell size, endogenous esterase activity, etc. Using the inhibitors and calculating the MAF…
Tue, Jul 18, 2023
Inconsistent fluorescence shift using the same cell (irreproducible results).
Inadequate incubation condition. Always use a water bath (not incubator) and ensure temperature of water bath is 37°C.
Tue, Jul 18, 2023
Do I need a luminometer with double injectors for this assay?
Yes. A single injector will not provide good results. You cannot pre-mix the arachidonic acid and substrate to make a…
Tue, Jul 18, 2023
Can I use cell culture samples in the COX Activity assay?
Cell culture samples are compatible with this kit. Since this is an activity assay rather than a quantitative immunoassay, there…
Tue, Jul 18, 2023
In the sPLA2 activity kit (ADI-907-002), how is a Unit defined?
One unit of phospholipase activity is the amount of enzyme needed to hydrolyze 1.0 µmole of L-a-phosphatidylcholine to L-a-lysophosphatidylcholine and…
Tue, Jul 18, 2023
DNA histograms are of poor overall quality.
The key elements in obtaining a histogram of high quality are good sample preparation, correct cell-to dye ratio and proper…
Tue, Jul 18, 2023