
Is the ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, compatible with the food proteins?
Beta-lactoglobulin is the control provided in the kit. In the food industry, protein aggregation is a desired attribute since aggregates impart…
Tue, Jul 18, 2023

I am having difficulty using my current lot of antibody for my intended application. What should I do?
Please provide as many details about the experiment as possible as well as an example of the data generated. Experiment details…
Tue, Jul 18, 2023

Cells do not exhibit fluorescence after incubation with the detection reagent.
Cell viability is low. Cells should be in log growth phase. Cell samples cannot be kept longer than 6 hours…
Tue, Jul 18, 2023

There are differences in MFI (mean fluorescence intensity) values between cell lines.
Detection reagent accumulation may be influenced by cell size, endogenous esterase activity, etc. Using the inhibitors and calculating the MAF…
Tue, Jul 18, 2023

Inconsistent fluorescence shift using the same cell (irreproducible results).
Inadequate incubation condition. Always use a water bath (not incubator) and ensure temperature of water bath is 37°C.
Tue, Jul 18, 2023

Fluorescence drop upon compound addition
Cells may become dislodged during addition. Decrease the rate of addition or seed fewer cells per well.
Tue, Jul 18, 2023

Chromatin condensation is not observed
Incorrect drug concentration or incubation time for cell type: Make sure the cell preparation protocol generates single cells with minimal…
Tue, Jul 18, 2023

Control cells without treatment show low orange signal.
Control cells are not healthy. Extended storage of cells after staining may adversely affect their health >> Use healthy cells…
Tue, Jul 18, 2023

Untreated cells have calcium response
Inconsistent DMSO concentration. Ensure that buffer used for the negative control wells has the same final concentration of DMSO as…
Tue, Jul 18, 2023

Mito-ID cell staining is too strong
Mitochondrial membrane potential dye is too concentrated for the cell type used. Optimize for different cell types by diluting the…
Tue, Jul 18, 2023