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How long can I use an ELISA kit after expiry?
Once ELISA kits are expired their performance can no longer be guaranteed. However, ELISA kits do not typically stop working entirely on their…
Tue, Jul 18, 2023
How should serum samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood in a covered test tube. If commercially available tubes are to be used, the researcher should use…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
How should samples be stored after preparation for the NGAL (human) ELISA kit?
Samples should be aliquoted and stored at +2-8 °C for immediate analysis, or frozen at -20 °C for analysis within…
Tue, Jul 18, 2023
Will my assay results vary depending on the sample type I am going to analyze with the NGAL (human) ELISA kit?
Analyses of samples of serum, EDTA plasma, citrate plasma, heparin plasma or urine showed no significant differences in analytical recovery,…
Tue, Jul 18, 2023
How can the high background be reduced?
During incubation with NBT/BCIP Reaction Mixture, localized blue-purple precipitate is formed. Decreasing the incubation time with NBT/BCIP Reaction Mixture can reduce the…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Progesterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023
Four-color IHC staining protocol
The protocol described below is a sequential staining process and may be adapted to any desired number of colors by adding…
Tue, Jul 18, 2023
What is the principle of the PKA kinase activity assay?
The PKA kinase activity kit allows relative quantification of PKA kinase activity in different sample types (purified, partially purified and…
Tue, Jul 18, 2023
Does the kit work for species other than human?
The ADI-EKS-390A kit is actually species independent; therefore, as long as there is sufficient PKA activity in your samples and…
Tue, Jul 18, 2023