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What is a good positive control to study protein aggregation in cells?
MG132 treated cells would serve as positive control to determine background noise compared to signal along with untreated negative control.
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with gram negative bacteria?
LPS in outer membrane of gram negative bacteria can be problematic. The permeabilizing buffer described in the manual, would remove…
Tue, Jul 18, 2023
Does your Protein A ELISA kit (ADI-900-057) detect both “natural” and “unnatural” Protein A?
As is illustrated in the figure included in the data sheet of ADI-900-057, this ELISA kit can detect both native…
Tue, Jul 18, 2023
Can ADI-EKS-750 detect human antibodies against DnaK, an E.coli analog of HSP70?
A similar assay has not been performed and therefore, a positive outcome of such experiment cannot be guaranteed. We would suggest using…
Tue, Jul 18, 2023
Which species, other than human, mouse or rat, can these HSP70 ELISA kits detect?
ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101 have not been tested with other species; so, it cannot be said with certainty whether it would be…
Tue, Jul 18, 2023
Which ELISA kit should I use to measure the levels of HSP70 in my serum/plasma samples?
The HSP70 ELISA kit (ADI-EKS-700B) was designed to measure HSP70 in cell and tissue lysates, and is not typically sensitive…
Tue, Jul 18, 2023
Which species can be analyzed with the LTB4 ELISA kit?
Leukotriene B4 (LTB4) is a small molecule and thus a defined chemical entity that does not change in between species…
Tue, Jul 18, 2023
Which C18 reverse phase columns can be used for sample extraction with the LTB4 ELISA kit?
The Honeywell Burdick & Jackson® Non-Polar Silica Based SPE Columns, Cat. No. 9002, is the recommended C18 reverse phase column for…
Tue, Jul 18, 2023