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Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023
What is the starting concentration of DNA needed for labeling with the CGH labeling kit for oligo arrays?
We suggest using 0.25 – 2.5 µg of input genomic DNA in a maximum volume of 19 µl, hence a…
Tue, Jul 18, 2023
How can the quality of DNA labeling by Enzo’s CGH labeling kits be analyzed?
We recommend using a NanoDrop UV-VIS Spectrophotometer in the microarray measurement mode to determine yield and dye incorporation. Use 1.5…
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
What is the detection mechanism used in your cellular senescence activity assay (ENZ-KIT129)?
Concerning the components of the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, the hypoxia detection reagent is relatively stable and can…
Tue, Jul 18, 2023
Is the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, specific for live cell imaging? Can cells be fixed after staining?
Yes, the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is validated on living cells only and tt would not be possible…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023
How many cells should I use with :your Cellular senescence activity assay?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Under what conditions were the cells subjected to create hypoxic conditions with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042? Were the cells grown under 95% N2 5 % CO2 ?
When validating the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, cells were grown in 95% nitrogen and also validated with either…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023