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Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023
Enzo suggests using 50 – 100 µg of Cot-1 DNA per sample when hybridizing the DNA labeled with the CGH labeling kits to Agilent arrays, is that correct?
The suggested amount of Cot-1 DNA in the protocols is correct. However, there is no theoretical reason why Enzo's kits…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
How can the quality of DNA labeling by Enzo’s CGH labeling kits be analyzed?
We recommend using a NanoDrop UV-VIS Spectrophotometer in the microarray measurement mode to determine yield and dye incorporation. Use 1.5…
Tue, Jul 18, 2023
How should I determine DNA concentration before labeling with Enzo’s CGH labeling kits?
We recommend determining concentration and purity of the genomic DNA using the nucleic acid module of a NanoDrop UV-VIS Spectrophotometer…
Tue, Jul 18, 2023
What is the starting concentration of DNA needed for labeling with the CGH labeling kit for oligo arrays?
We suggest using 0.25 – 2.5 µg of input genomic DNA in a maximum volume of 19 µl, hence a…
Tue, Jul 18, 2023
Does genomic DNA need to be fragmented for labeling with Enzo’s CGH labeling kits?
Restriction digestion or fragmentation of the isolated genomic DNA is not required prior to labeling with our CYTAG® CGH (ENZ-42671)…
Tue, Jul 18, 2023
What is the required fragment length of input DNA for labeling with Enzo’s CGH labeling kits?
Comparative genome hybridization (CGH) does not require DANN fragmentation prior to analysis and can be performed with genomic input DANN.…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023