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What will be the effect of decreasing the labeling time with Enzo’s CGH labeling kits below the recommended 4 hours?
For input DNA of good quality and quantity, shorter labeling times down to 2 hours may be sufficient, but the…
Tue, Jul 18, 2023
What is the optimal time for hybridization of DNA that was labeled with Enzo’s CGH labeling kits?
Please follow the recommendations of the CGH array manufacturer you are using.
Tue, Jul 18, 2023
How should the Cyanine dyes of Enzo’s CGH labeling kits be stored after thawing?
For up to ten reactions, they can directly be refrozen at -20°C in the dark. For more reactions, they should…
Tue, Jul 18, 2023
Do I really need to add Stop Buffer after the labeling reaction with Enzo’s CGH labeling kits even though the enzyme is heat-inactivated?
During kit validation, a protocol utilizing a 4-hour labeling step and addition of Stop Buffer gave us the best results…
Tue, Jul 18, 2023
For the Protein A ELISA kit, ADI-900-057, would the readings be affected if the wells were to dry out when residual wash buffer is blotted out?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), care should be taken not to allow the…
Tue, Jul 18, 2023
For the Protein A ELISA kit (ADI-900-057) would residual wash buffer affect the assay?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), residual wash buffer can interfere with the assay…
Tue, Jul 18, 2023
Does the Protein A ELISA kit recognize denatured and non-denatured protein A?
A variety of Protein A constructs have been tested in our Protein A ELISA kit. Please refer to the product…
Tue, Jul 18, 2023
Can the BIOARRAY™ Terminal labeling kit (ENZ-42630) be used to label sequences of 100 nucleotides?
The BIOARRAYTM Terminal labeling kit kit was optimized with a 30-mer at 1 µg or 100 pmol, but a longer…
Tue, Jul 18, 2023
How does the BIOARRAY™ Terminal labeling kit, ENZ-42630, label nucleotides?
When using the BIOARRAYTMTerminal labeling kit, the Terminal Deoxynucleotide Transferase catalyzes the additional single biotin-ddUMP to the 3’-OH terminus of…
Tue, Jul 18, 2023
Is the CYTAG™ CGH Labeling kit also suitable for BAC arrays?
The CYTAG™ CGH Labeling kit was designed for the more demanding high density oligo arrays, but it also works well…
Tue, Jul 18, 2023