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How can I improve the intensity of the signal obtained with the LEADING LIGHT® Wnt reporter cells?
Conditions in terms of cell density, culture medium, and incubation have to be respected. It is very important that there…
Tue, Jul 18, 2023
How long should I incubate my LEADING LIGHT® Wnt reporter cells after adding the luciferase substrate?
Signals should be read 10 minutes after adding the luciferase substrate with a multichannel pipette. The signal is stable enough…
Tue, Jul 18, 2023
Can components of Enzo’s CGH labeling kits be purchased separately?
Unfortunately, we will not provide components of our CGH labeling kits as our kits are readily packaged and QCed together.…
Tue, Jul 18, 2023
Can you share the recipe of the Cell Growth Medium?
This information is considered to be proprietary and we cannot disclose the actual recipe. It contains additives and nutrients. These…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
What is the optimal time for hybridization of DNA that was labeled with Enzo’s CGH labeling kits?
Please follow the recommendations of the CGH array manufacturer you are using.
Tue, Jul 18, 2023
What will be the effect of decreasing the labeling time with Enzo’s CGH labeling kits below the recommended 4 hours?
For input DNA of good quality and quantity, shorter labeling times down to 2 hours may be sufficient, but the…
Tue, Jul 18, 2023
In which cases do you recommend to digest the DNA prior to labeling with Enzo’s CGH labeling kits?
For CGH+SNP arrays, genomic DNA must be digested using a combination of the restriction enzymes AluI and RsaI. For CGH…
Tue, Jul 18, 2023
Apart from the labeling kits, which ancillary reagents does Enzo offer for CGH?
Enzo does provide reagents for restriction, labeling and purification of DNA and Cot DNA. Furthermore, ENZO provides control Human Genomic…
Tue, Jul 18, 2023
Can I use the LEADING LIGHT® Wnt reporter cells straight from the liquid nitrogen?
We would advise against using the cells from the liquid nitrogen. Cells need to be in their exponential growing phase…
Tue, Jul 18, 2023