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Can multiple targets be detected with ISH?
Yes, it is common practice to stain with multiple ISH probes in parallel. However, while continuously-labeled immunofluorescent probes can be…
Tue, Jul 18, 2023
How can slides be stored after ISH?
That depends mostly on fixation/embedding method and the type of ISH performed. After chromogenic ISH on FFPE or cryopreserved tissue,…
Tue, Jul 18, 2023
Why should post-hybridization washes be carried out?
Post-hybridization washing after ISH will reduce non-specific signal by removing probes that are lightly bound to off-target sequences. Most frequently,…
Tue, Jul 18, 2023
Why are the Gold 525 dUTPs bleeding through in the red channel?
If an epifluorescence microscope is used for visualization, the incorporated dUTPs should be matched to the filters equipped in the…
Tue, Jul 18, 2023
Why are Aqua 431 dUTP-labeled probes much harder to detect?
There are two aspects making Aqua-labeled probes harder to detect: First, the aqua fluorophore (DEAC – 7-Diethylaminocoumarin-3-carboxylic acid) has a…
Tue, Jul 18, 2023
How to cover up the sections during hybridization in ISH protocols?
The most common method is to cover tissue sections on glass slides in the hybridization solution with a glass cover…
Tue, Jul 18, 2023
Is protein blocking really necessary in ISH?
Blocking with bovine serum albumin (BSA), milk or serum is always recommended when ISH signals are detected by antibodies (e.g.…
Tue, Jul 18, 2023
Why is peroxidase blocking necessary in colorimetric ISH/IHC detection?
When colorimetric detection by enzymatic reaction is used, a blocking step with peroxide solution is required to eliminate endogenous peroxidase…
Tue, Jul 18, 2023
How important are denaturation and hybridization temperature in ISH?
Correct denaturation and hybridization temperatures are paramount for successful ISH. Regardless of the probe or target being RNA or DNA,…
Tue, Jul 18, 2023
What are the advantages of digoxigenin labeling?
For chromogenic ISH, Digoxigenin, compared to biotin, offers the advantage of lower background, higher specificity. Is also possible to combine…
Tue, Jul 18, 2023