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Organelle Detection
How long is the NUCLEAR-ID® red dye (ENZ-52406) retained by the cells and is the dye toxic to the cells?
NUCLEAR-ID® red DNA stain ( ENZ-52406) is fairly stable. We do not have specifics on how long the dye would be…
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?
No, these are two different dyes.
Tue, Jul 18, 2023
How specific is the NUCLEAR-ID® red dye (ENZ-52406) for dsDNA? What is the binding capacity of the dye to ssRNA, ssDNA and DNA:RNA Hybrids?
The dye stains dsDNA. Data is not available for binding capacity to ssRNA, ssDNA and DNA:RNA hybrids.
Tue, Jul 18, 2023
Control cells without treatment show low orange signal.
Control cells are not healthy. Extended storage of cells after staining may adversely affect their health >> Use healthy cells…
Tue, Jul 18, 2023
Poor Mito-ID staining observed.
Stained cells may have been exposed to strong light. Mito-ID® MP dye is light sensitive; be sure to protect samples…
Tue, Jul 18, 2023
Mito-ID cell staining is too strong
Mitochondrial membrane potential dye is too concentrated for the cell type used. Optimize for different cell types by diluting the…
Tue, Jul 18, 2023
The dyes fail to stain acidic organelles in fixed and/or permeabilized cells.
The following kits are only suitable for live cell staining only. Use them only for live cell analysis. Cyto-ID® Autophagy…
Tue, Jul 18, 2023
Organelles are not sufficiently stained.
Very low concentration of dye was used, or dye was incubated with the cells for an insufficient length of time.…
Tue, Jul 18, 2023
Nuclear counterstain is too bright compared to the organelle stain.
Different microscopes, cameras and filters may make some signals appear very bright. Reduce the concentration of the nuclear counterstain or…
Tue, Jul 18, 2023