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Life Sciences
How soon after incubation with Cyto-ID® autophagy dye would the samples need to be analyzed by flow cytometry? The user manual recommends following 30 minutes incubation. Is it advisable to temporarily store samples on ice before they are analyzed?
One can store the samples on ice for a short period of time to extend the period of optimal staining.…
Tue, Jul 18, 2023
Can GFP-Certified™ Apoptosis/Necrosis Detection Kit, ENZ-51002, and Cyto-ID® Autophagy Detection Kit, ENZ-51031, be combined to perform triple staining simultaneously detecting apoptotic, necrotic and autophagic cells ?
GFP-Certified™ Apoptosis/Necrosis Detection Kit, ENZ-51002, and Cyto-ID®Autophagy Detection Kit, ENZ-51031, cannot be used in combination because of spectral overlap between…
Tue, Jul 18, 2023
How do the ROS/RNS dyes work?
Nitric Oxide (Red dye): The strategy for fluorescence-based detection of NO employs an o-phenylenediamine scaffold, which in the presence of…
Tue, Jul 18, 2023
For the ROS/RNS detection kit, ENZ-51001, can samples be stored after the final wash buffer step in the protocol, and do the microscopy at a later date?
No, this is a vital stain. Live cells must be read immediately per the manual.
Tue, Jul 18, 2023
Using this kit, is it necessary to wash the cells after staining?
No, the kit does not require a wash step.
Tue, Jul 18, 2023
How long can the fluorescence signal be detected in live cells?
Typically, cells are induced to undergo autophagy and then stained just prior to analysis. Induction can be as little as…
Tue, Jul 18, 2023
What is the Kd and quantum yield of FluoForte® Calcium Assay kit?
Kd is estimated to be 389 nM (22°C, pH 7.0 –7.5). Maximum quantum yield is 0.18 at pH 7.2.
Tue, Jul 18, 2023
Good DLRs in the CGH analytics QC report, but some oligos show whiskering or significant deviation from the expected log2 ratio
We suggest keeping the Cot-1 DNA at 50 µg regardless of the hybridization volume.
Tue, Jul 18, 2023
Poor signal to noise ratio despite good incorporation
Low signal can be caused by inappropriate hybridization conditions (too stringent buffer or excessive temperature) or when the hybridization time…
Tue, Jul 18, 2023
*General Information
The solubility of small molecule products, especially those that are hydrophobic can sometimes be a limiting factor in experimental design.…
Tue, Jul 18, 2023