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How much sample do I need to use for cAMP/cGMP quantification and what are the suggested dilutions?
The amount of sample required and the dilutions needed for the assay depend upon the amount of cAMP/cGMP in the…
Tue, Jul 18, 2023
What if my cells are not sufficiently lyzed for cAMP/cGMP quantification after the addition of 0.1M HCl?
Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used…
Tue, Jul 18, 2023
Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023
Which protein assay is compatible with lysates obtained with HCl treatment?
This question is actually better suited for a company supplying the protein assay you wish to use. Thermo, for example,…
Tue, Jul 18, 2023
How much Assay Buffer is required to reconstitute my serum samples in prior to cAMP/cGMP quantification?
The volume of Assay Buffer needed to reconstitute samples is completely dependent on experimental conditions. We recommend re-suspending 1mL of…
Tue, Jul 18, 2023
How many cells should I work with for the quantification of cAMP or cGMP?
The amount of tissue or number of cells to use is entirely dependent on the experimental conditions. It is recommended…
Tue, Jul 18, 2023
Can I store acetylated samples for cAMP/cGMP quantification at a later time?
No. We recommend that only enough samples needed for the current run be acetylated. Samples are more stable when stored…
Tue, Jul 18, 2023
Is the acetylation step really necessary prior to cAMP/cGMP quantification?
Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is…
Tue, Jul 18, 2023
Can any culture medium be used to measure cAMP/cGMP in culture supernatants with cAMP/cGMP ELISA and cAMP/cGMP Complete ELISA kits?
As indicated in the insert, as long as the standard curve is titrated in the same culture media that the…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP ELISA kit be used with the cGMP ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023