Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Life Sciences
My first assay for total glutathione worked well, but when I repeated the assay to test the oxidized samples, the curve was essentially flat-to-negative.
Oxidized glutathione is very difficult to detect because it is rapidly turned over and converted, so there is only a…
Tue, Jul 18, 2023
After having measured the total glutathione content, is it possible to use the same samples on the plate so as to continue the procedure and measure the oxidized form of glutathione?
The measurements of total and oxidized glutathione are two completely different events; it is therefore not possible to use the…
Tue, Jul 18, 2023
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023
What is the purpose of treating samples with 5% metaphosphoric acid (MPA)?
MPA should be added to fresh samples prior to sample handling or sample storage. That is required to deproteinize samples…
Tue, Jul 18, 2023
What is the principle of the Glutathione (GSSG/GSH) assay?
The Glutathione (total), detection kit is a complete kit that allows the determination of total, reduced, and oxidized glutathione concentrations…
Tue, Jul 18, 2023
Will the cytokine ELISA kits from one species work with another species?
This will depend on the species homology. Human cytokine ELISA kits will generally not measure cytokines in other species. Mouse…
Tue, Jul 18, 2023
What antibody pairs are used in the kit?
This varies from kit to kit. Refer to the product specific instruction manual for more information.
Tue, Jul 18, 2023
What species can the eicosanoid ELISA kits be used for?
Eicosanoids are not species-specific. However, the antisera used in a particular assay may present an interference effect due to the…
Tue, Jul 18, 2023
When using an eicosanoid ELISA kit, how much indomethacin should I use in my samples?
You should follow the suggestion in the product specific manual, and add up to 10µg per ml of liquid sample.…
Tue, Jul 18, 2023
Do eicosanoid assays require an extraction?
The majority of samples do not require an extraction. However, in some situations, an extraction will be required when the…
Tue, Jul 18, 2023