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Life Sciences
What detergents are compatible with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027 and ProteoStat® Protein Aggregation Assay?
The ProteoStat® dye is compatible with some detergents, such as 0.1% Tween 20, but this varies with the protein used. …
Tue, Jul 18, 2023
Are there any limitations regarding particle size of the aggregates that can be detected with the ProteoStat® dye?
The ProteoStat® dye detects in the subvisible to visible range from ~ 50 µm to 1 mm.
Tue, Jul 18, 2023
What does ProteoStat® dye bind to and what is the mechanism of detection of protein aggregation?
The molecular rotor dye is non-fluorescent due to free rotation around a central carbon-carbon single bond separating different aromatic portions of…
Tue, Jul 18, 2023
How does the steroid displacement reagent (SDR) work?
Steroids in circulation are almost all ionically bound to carrier proteins such as albumin or more specific binding proteins. Treating…
Tue, Jul 18, 2023
Why is there sample dilution necessary in this ELISA Kit?
There are several reasons for dilutions. In some cases samples might read above the standard curve, thus requiring a dilution…
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023
Would the aggregation temperature as determined using ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, be the same as determined using the Thermofluor technique?
In Thermofluor technique, SYPRO Orange, an environment-sensitive dye, is used to measure the unfolding of the protein as the temperature…
Tue, Jul 18, 2023
Is it possible to distinguish between protein aggregates of different sizes using ProteoStat® Protein Aggregation Assay, ENZ-51023?
The ProteoStat® detection dye does not differentiate aggregate sizes. The dye will bind to a wide range of protein aggregate…
Tue, Jul 18, 2023
Can DNA labeled with the Enzo Life Sciences kit be used on a Roche NimbleGen™ platform?
Yes, it can be used. It has been noted by cytogenetics labs that Enzo’s CGH labeling kit works perfectly well…
Tue, Jul 18, 2023
What are the critical points to pay attention to during preparation of ß-Lactoglobulin solution?
During preparation of ß-Lactoglobulin solution, the following points should be noted: The ß-Lactoglobulin Control is supplied as a lyophilized powder…
Tue, Jul 18, 2023