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Which is the best dilution for my sample type?
In the manual, section “Sample recoveries”, we provide a list of recommended dilutions. Those are the dilutions our development scientists…
Tue, Jul 18, 2023
How can I overcome sample matrix interference?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine…
Tue, Jul 18, 2023
Do you sell positive controls that can be run with your steroid ELISA kits?
We do not provide positive control samples with our kits. If you need to ensure accurate recovery of any analyte…
Tue, Jul 18, 2023
Are there any limitations regarding particle size of the aggregates that can be detected with the ProteoStat® dye?
The ProteoStat® dye detects in the subvisible to visible range from ~ 50 µm to 1 mm.
Tue, Jul 18, 2023
How should I test for possible matrix interference with my samples?
Matrix interference describes the situation where the sample matrix (buffer salts, pH, additives like detergents or solvents, proteins, …) interferes…
Tue, Jul 18, 2023
What are the standard QC parameters this kit must accommodate?
For a typical labeling reaction with an input of 500 ng of high quality genomic DNA, the yield should be…
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023
Can the ProteoStat® TS Detection Reagent in the thermal shift stability assay kit, ENZ-51027, be substituted with the detection reagent from the ProteoStat® Protein Aggregation Assay, ENZ-51023?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
Would the aggregation temperature as determined using ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, be the same as determined using the Thermofluor technique?
In Thermofluor technique, SYPRO Orange, an environment-sensitive dye, is used to measure the unfolding of the protein as the temperature…
Tue, Jul 18, 2023
Is the binding of the protein to the ProteoStat® detection dye reversible?
The interaction between the ProteoStat® dye and the protein aggregates is non-covalent. So, in theory it is a reversible interaction.…
Tue, Jul 18, 2023