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Life Sciences
How should I determine DNA concentration before labeling with Enzo’s CGH labeling kits?
We recommend determining concentration and purity of the genomic DNA using the nucleic acid module of a NanoDrop UV-VIS Spectrophotometer…
Tue, Jul 18, 2023
Enzo suggests using 50 – 100 µg of Cot-1 DNA per sample when hybridizing the DNA labeled with the CGH labeling kits to Agilent arrays, is that correct?
The suggested amount of Cot-1 DNA in the protocols is correct. However, there is no theoretical reason why Enzo's kits…
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
Immunohistochemistry protocol validated for these antibodies
Immunohistochemistry protocol validated for the antibodies described below: ADI-905-781, ADI-KAP-GP005, ADI-VAM-PT046, ADI-VAM-SV016, ADI-VAM-SV020, ADI-VAM-SV021, ADI-VAM-SV026, ADI-VAP-PT049, ADI-VAP-SV065, ADI-AAP-113, ADI-CSA-815, ADI-OSA-110,…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Are collagen-coated plates compatible with your cellular senescence activity assay (ENZ-KIT129)?
Collagen will not interfere with this assay. You can, therefore, grow your cells on collagen-coated plates.
Tue, Jul 18, 2023
Can ENZ-KIT130 be used with cells expressing GFP?
ENZ-KIT130 is not recommended for use on GFP-expressing cells because of the emission spectra overlap between GFP and the fluorogenic…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023
How many cells should I use with :your Cellular senescence activity assay?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Are ENZ-KIT129 and ENZ-KIT130 compatible with any cell line?
The SA-b-galactosidase is a universal marker for any senescent cell and this assay is not cell type-specific.
Tue, Jul 18, 2023