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Does the Protein A ELISA kit recognize denatured and non-denatured protein A?
A variety of Protein A constructs have been tested in our Protein A ELISA kit. Please refer to the product…
Tue, Jul 18, 2023
For the Protein A ELISA kit (ADI-900-057) would residual wash buffer affect the assay?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), residual wash buffer can interfere with the assay…
Tue, Jul 18, 2023
Is there a way to quickly check for the labeling efficiency of Enzo’s CGH labeling kits?
Visual inspection after combining the Cyanine 3-labeled and Cyanine 5-labeled and cleaned DNA should be visibly purple. More detailed analysis…
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be handled?
We recommend aliquoting the kit reagents upon first use into volumes convenient for your workflow, and to avoid repeated freezing…
Tue, Jul 18, 2023
Is the CYTAG™ CGH Labeling kit also suitable for BAC arrays?
The CYTAG™ CGH Labeling kit was designed for the more demanding high density oligo arrays, but it also works well…
Tue, Jul 18, 2023
How does the BIOARRAY™ Terminal labeling kit, ENZ-42630, label nucleotides?
When using the BIOARRAYTMTerminal labeling kit, the Terminal Deoxynucleotide Transferase catalyzes the additional single biotin-ddUMP to the 3’-OH terminus of…
Tue, Jul 18, 2023
Can the BIOARRAY™ Terminal labeling kit (ENZ-42630) be used to label sequences of 100 nucleotides?
The BIOARRAYTM Terminal labeling kit kit was optimized with a 30-mer at 1 µg or 100 pmol, but a longer…
Tue, Jul 18, 2023
I expect calcineurin to be mainly located in the nuclei. How should I treat my cell lysates in order to avoid loss of nuclei whe using Calcineurin cellular activity assay kit, BML-AK816?
You need to replace the high speed centrifugation suggested in the Calcineurin cellular activity assay kit (BML-AK816) manual which pellets…
Tue, Jul 18, 2023
Can the desalting resin provided with the kit BML-AK816, Calcineurin cellular activity assay kit be reused?
Yes, the desalting resin provided with the Calcineurin cellular activity assay kit (BML-AK816) can be reused for multiple samples. Between…
Tue, Jul 18, 2023
In the manual for the Calcineurin cellular activity assay kit, BML-AK816, you recommend using 5,000-50,000 cells per assay point. How much lysis buffer should be used to prepare the samples?
it is easier to dilute samples than to concentrate them. In order to prepare cells for use with Calcineurin cellular…
Tue, Jul 18, 2023