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Genomic Analysis
What are the key advantages of Enzo’s CGH labeling kits in comparison to the kits Agilent offers?
All our CGH labeling kits outperform the kits offered by Agilent for labeling: They provide excellent DNA yields with superior…
Tue, Jul 18, 2023
What are the molar extinction coefficients of the Cyanine 3 and Cyanine 5 dyes in Enzo’s CGH labeling kits?
Cyanine 3: 150,000 M cm-1; Cyanine 5: 250,000 M cm-1
Tue, Jul 18, 2023
Is there a way to quickly check for the labeling efficiency of Enzo’s CGH labeling kits?
Visual inspection after combining the Cyanine 3-labeled and Cyanine 5-labeled and cleaned DNA should be visibly purple. More detailed analysis…
Tue, Jul 18, 2023
How have the results you show on a patient with Prader-Willi syndrome been generated?
These results have been generated by Enzo scientists using the CYTAG™ CGH Labeling kit on an Agilent 4X180K oligonucleotide microarray.
Tue, Jul 18, 2023
What is the difference between low- and high-density arrays?
Low-density arrays are usually prepared on glass slides with an average density of about 102 spots per cm2. High-density arrays…
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023
Enzo suggests using 50 – 100 µg of Cot-1 DNA per sample when hybridizing the DNA labeled with the CGH labeling kits to Agilent arrays, is that correct?
The suggested amount of Cot-1 DNA in the protocols is correct. However, there is no theoretical reason why Enzo's kits…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
How can the quality of DNA labeling by Enzo’s CGH labeling kits be analyzed?
We recommend using a NanoDrop UV-VIS Spectrophotometer in the microarray measurement mode to determine yield and dye incorporation. Use 1.5…
Tue, Jul 18, 2023