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Genomic Analysis
When I opened the parcel containing the CGH labeling kit from Enzo, all of the dry ice had disappeared, will it still be good to use?
If the vials in the kit were still frozen when you received them then the product is okay to use.…
Tue, Jul 18, 2023
Is the CYTAG™ CGH Labeling kit also suitable for BAC arrays?
The CYTAG™ CGH Labeling kit was designed for the more demanding high density oligo arrays, but it also works well…
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be handled?
We recommend aliquoting the kit reagents upon first use into volumes convenient for your workflow, and to avoid repeated freezing…
Tue, Jul 18, 2023
What are the key advantages of Enzo’s CGH labeling kits in comparison to the kits Agilent offers?
All our CGH labeling kits outperform the kits offered by Agilent for labeling: They provide excellent DNA yields with superior…
Tue, Jul 18, 2023
What are the molar extinction coefficients of the Cyanine 3 and Cyanine 5 dyes in Enzo’s CGH labeling kits?
Cyanine 3: 150,000 M cm-1; Cyanine 5: 250,000 M cm-1
Tue, Jul 18, 2023
Is there a way to quickly check for the labeling efficiency of Enzo’s CGH labeling kits?
Visual inspection after combining the Cyanine 3-labeled and Cyanine 5-labeled and cleaned DNA should be visibly purple. More detailed analysis…
Tue, Jul 18, 2023
How should the Cyanine dyes of Enzo’s CGH labeling kits be stored after thawing?
For up to ten reactions, they can directly be refrozen at -20°C in the dark. For more reactions, they should…
Tue, Jul 18, 2023
How long can Enzo’s CGH labeling kits be stored after first use?
Independent of the date of first use we can only guarantee for performance of Enzo's CGH labeling kits for up…
Tue, Jul 18, 2023
Do I really need to add Stop Buffer after the labeling reaction with Enzo’s CGH labeling kits even though the enzyme is heat-inactivated?
During kit validation, a protocol utilizing a 4-hour labeling step and addition of Stop Buffer gave us the best results…
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be stored?
Upon receipt, all reagents of the kit should be stored at –20 °C.
Tue, Jul 18, 2023