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General Questions
Paraformaldehyde/Glutaraldehyde Fixation
Paraformaldehyde/glutaraldehyde fixation(method of choice for double labeling of membrane bound and cytoskeletal antigens) Rinse cells with PBS at room temperature.…
Tue, Jul 18, 2023
Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023
Immunohistochemistry Protocol
Protocols for immunohistochemistry vary widely due to differences in antigens and their recognition by antibodies. Various fixation methods can uncover…
Tue, Jul 18, 2023
Applications
Abbreviation Full Name AA Activity Assay AC Affinity Chromatography CIE Crossed Immune Electrophoresis DB Dot Blot…
Tue, Jul 18, 2023
Antibody Conjugation
Abbreviation Full Name PE R-Phycoerythrin DY DYTM is a trademark of the Dyomics , Inc. DyLightTM 488 …
Tue, Jul 18, 2023
General Abbreviations
Abbreviation Full Name mAb Monoclonal Antibody pAb Polyclonal Antibody cAb Clonal Antibody CT C-terminus NT …
Tue, Jul 18, 2023
General Immunoprecipitation Protocol
Immunoprecipitation (IP) is a common useful immunochemical technique. When coupled with SDS-PAGE, IP can reveal a number of important characteristics…
Tue, Jul 18, 2023