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General Questions
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Immunohistochemistry Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membrane Preparation Protocol
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membranes ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Blocking Peptide Neutralization
Reconstitute the lyophilized peptide with 0.1 mL distilled water or PBS. Be careful to reconstitute entire contents of vial as…
Tue, Jul 18, 2023
Western Blot Protocol
1. Transfer proteins from gel to membrane (nitrocellulose or PVDF). Gloves should be worn when handling gels or blot membrane.…
Tue, Jul 18, 2023
Immunoprecipitation of Phosphoserine-, Phosphothreonine- and Phosphotyrosine-Containing Proteins
Important Remarks: Rinse all plastic vessels with BSA solution (10 mg/mL in lysis buffer). Use lipid-free BSA, e.g., Sigma A7906. For…
Tue, Jul 18, 2023
Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Cell ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note:The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not been…
Tue, Jul 18, 2023
Paraformaldehyde/Glutaraldehyde Fixation
Paraformaldehyde/glutaraldehyde fixation(method of choice for double labeling of membrane bound and cytoskeletal antigens) Rinse cells with PBS at room temperature.…
Tue, Jul 18, 2023