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Membranes ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Blocking Peptide Neutralization
Reconstitute the lyophilized peptide with 0.1 mL distilled water or PBS. Be careful to reconstitute entire contents of vial as…
Tue, Jul 18, 2023
Immunoprecipitation of Phosphoserine-, Phosphothreonine- and Phosphotyrosine-Containing Proteins
Important Remarks: Rinse all plastic vessels with BSA solution (10 mg/mL in lysis buffer). Use lipid-free BSA, e.g., Sigma A7906. For…
Tue, Jul 18, 2023
Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Western Blot Protocol
1. Transfer proteins from gel to membrane (nitrocellulose or PVDF). Gloves should be worn when handling gels or blot membrane.…
Tue, Jul 18, 2023
Cell ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note:The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not been…
Tue, Jul 18, 2023
Competitive ELISA Assay
For small molecule analytes, typically less than 10 kDa, the competitive ELISA Kit is used. An advantage of this assay…
Tue, Jul 18, 2023
Checking for Interference or Linearity
Interference is when some component of a sample prevents the appropriate binding events to take place. Percent recovery is the…
Tue, Jul 18, 2023
Methanol Fixation
Methanol fixation (for cytoskeletal components) The methanol fixation is an easy method. However, it frequently solubilizes and removes membrane bound…
Tue, Jul 18, 2023