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Enzyme Activity Assays
Are results from Creatinine colorimetric detection kit, ADI-907-030A and Creatinine colorimetric detection kit, ADI-907-030 comparable?
Data obtained from Creatinine colorimetric detection kit, ADI-907-030A and Creatinine colorimetric detection kit, ADI-907-030 are comparable for the same set…
Tue, Jul 18, 2023
What is the recommended concentration of MG132, BML-PI102 used in proteasome research?
Recommend concentrations of MG132, BML-PI102 typically ranges from 10 uM to 150 uM and is cell dependent. We have not established…
Tue, Jul 18, 2023
In the reformulated BIOMOL® Green, BML-AK111, Tergitol15-S-9 replaces NP-40 detergent. While NP-40 is nonionic (no charge), would the switch cause any change in the overall properties or reaction rates?
Tergitol15-S-9 is also non-ionic in nature and this switch from a chemically similar detergent should not affect rates of reaction…
Tue, Jul 18, 2023
Would the results obtained with Creatinine colorimetric detection kit, ADI-907-030 and Creatinine colorimetric detection kit, ADI-907-030A be identical?
Results obtained with old and new version kit would not be identical. But, the values would be close and comparable…
Tue, Jul 18, 2023
What buffer should be used to dilute standards, ATP and active PKC in ADI-EKS-420A, PKC Kinase Activity Assay Kit?
Only Kinase Assay Dilution Buffer (80-1486) provided with PKC kinase activity kit (ADI-EKS-420A) should be used to dilute ATP, Standards…
Tue, Jul 18, 2023
What volume of recombinant PKC or cell extracts should be used with ADI-EKS-420A?
In experiments with mouse brain lysates using the PKC kinase activity kit (ADI-EKS-420A), we found that 0.02 – 2µg of…
Tue, Jul 18, 2023
What is the difference between Calcineurin Phosphatase assay kit, BML-AK804 and Calcineurin cellular activity assay kit, BML-AK816?
Calcineurin Phosphatase assay kit (BML-AK804) is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. This kit comes with…
Tue, Jul 18, 2023
Which PKC isoform is supplied as the active enzyme in the PKC kinase activity kit, ADI-EKS-420A?
The PKC kinase activity kit (ADI-EKS-420A) contains recombinant PKCµ as positive control.
Tue, Jul 18, 2023
What starting volume is required for COX activity assay kit, ADI-907-003?
For COX activity assay kit, ADI-907-003 we recommend starting volumes of 50 µL COX-I or COX-II preparations. Actual sample volumes required…
Tue, Jul 18, 2023
Can some guidance be provided to detect the activity of COX1 and COX2 from human plasma, and advise assay design with the Ibuprofen, meloxicam, known COX-2 inhibitors for COX activity assay kit, ADI-907-003?
We have not specifically validated this kit with human plasma samples. Page 5 of user manual provides the work flow. This provides…
Tue, Jul 18, 2023