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ELISA Immunoassays
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the incubation times or temperatures? Do I need to shake my plate?
The assay procedure has been optimized, and any alterations will require validation.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how long can I store the components after I reconstitute them?
The reconstituted reagents should be aliquotted and stored at the temperature recommended in the instruction manual for up to three…
Tue, Jul 18, 2023
What protocol can be used for drying samples prior to cAMP/cGMP quantification?
Samples can be dried using either vacuum centrifugation (e.g. SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will the type of plates I use for coating affect the assay performance?
We recommend using plates made with high binding plastic, similar to Corning plates (2592). The use of different types of…
Tue, Jul 18, 2023
How much Assay Buffer is required to reconstitute my serum samples in prior to cAMP/cGMP quantification?
The volume of Assay Buffer needed to reconstitute samples is completely dependent on experimental conditions. We recommend re-suspending 1mL of…
Tue, Jul 18, 2023
Which protein assay is compatible with lysates obtained with HCl treatment?
This question is actually better suited for a company supplying the protein assay you wish to use. Thermo, for example,…
Tue, Jul 18, 2023
How much sample do I need to use for cAMP/cGMP quantification and what are the suggested dilutions?
The amount of sample required and the dilutions needed for the assay depend upon the amount of cAMP/cGMP in the…
Tue, Jul 18, 2023
I am not getting consistent results with my cAMP/cGMP quantification in cells. I think that my cells may be permeabilized.
For the most consistent results, we recommend that cells be fully disrupted before using the lysate in an assay. Disruption…
Tue, Jul 18, 2023
My maximum binding for acetylated cAMP/cGMP standards and samples is only about 50%. Why?
This occurs if the standards and samples were acetylated but the B0 standard was not. Your data can still be…
Tue, Jul 18, 2023