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ELISA Immunoassays
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the incubation times or temperatures? Do I need to shake my plate?
The assay procedure has been optimized, and any alterations will require validation.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how long can I store the components after I reconstitute them?
The reconstituted reagents should be aliquotted and stored at the temperature recommended in the instruction manual for up to three…
Tue, Jul 18, 2023
My maximum binding for acetylated cAMP/cGMP standards and samples is only about 50%. Why?
This occurs if the standards and samples were acetylated but the B0 standard was not. Your data can still be…
Tue, Jul 18, 2023
I am not getting consistent results with my cAMP/cGMP quantification in cells. I think that my cells may be permeabilized.
For the most consistent results, we recommend that cells be fully disrupted before using the lysate in an assay. Disruption…
Tue, Jul 18, 2023
What protocol can be used for drying samples prior to cAMP/cGMP quantification?
Samples can be dried using either vacuum centrifugation (e.g. SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023
Can I measure the levels of cAMP/cGMP in rabbit sera considering that the plate is coated with an anti-rabbit IgG?
The plate provided in these kits is coated with a generic antibody capable of capturing the cAMP- or cGMP-specific antibody.…
Tue, Jul 18, 2023
What are the expected concentrations of cAMP/cGMP in my samples?
The amounts of cAMP and cGMP in samples depend on sample type, origin, or even treatment. Unfortunately, this is not…
Tue, Jul 18, 2023
Can I use the cAMP/cGMP ELISA kits for agonism and antagonism studies?
As long as the experimental design is structured so that the end samples can be incorporated into the assay as…
Tue, Jul 18, 2023
How much sample do I need to use for cAMP/cGMP quantification and what are the suggested dilutions?
The amount of sample required and the dilutions needed for the assay depend upon the amount of cAMP/cGMP in the…
Tue, Jul 18, 2023