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ELISA Immunoassays
When using an IMMUNOSET® ELISA development set, can I use a different buffer to dilute my standards?
Use of buffers other than those recommended will require validation by the user.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use other Streptavidin-HRP solutions?
Yes, however the reagent should be optimized (titered) to assure acceptable signal intensity and background are achieved.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, what kind of substrate should I use to develop the plate?
Any colorimetric peroxidase substrate compatible with the enzyme of choice as well as your plate reader should be acceptable. See…
Tue, Jul 18, 2023
My maximum binding for acetylated cAMP/cGMP standards and samples is only about 50%. Why?
This occurs if the standards and samples were acetylated but the B0 standard was not. Your data can still be…
Tue, Jul 18, 2023
I am not getting consistent results with my cAMP/cGMP quantification in cells. I think that my cells may be permeabilized.
For the most consistent results, we recommend that cells be fully disrupted before using the lysate in an assay. Disruption…
Tue, Jul 18, 2023
What protocol can be used for drying samples prior to cAMP/cGMP quantification?
Samples can be dried using either vacuum centrifugation (e.g. SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023
Can I measure the levels of cAMP/cGMP in rabbit sera considering that the plate is coated with an anti-rabbit IgG?
The plate provided in these kits is coated with a generic antibody capable of capturing the cAMP- or cGMP-specific antibody.…
Tue, Jul 18, 2023
What are the expected concentrations of cAMP/cGMP in my samples?
The amounts of cAMP and cGMP in samples depend on sample type, origin, or even treatment. Unfortunately, this is not…
Tue, Jul 18, 2023
How much sample do I need to use for cAMP/cGMP quantification and what are the suggested dilutions?
The amount of sample required and the dilutions needed for the assay depend upon the amount of cAMP/cGMP in the…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will the type of plates I use for coating affect the assay performance?
We recommend using plates made with high binding plastic, similar to Corning plates (2592). The use of different types of…
Tue, Jul 18, 2023