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ELISA Immunoassays
When using an IMMUNOSET® ELISA development set, can I prepare a different standard range than what is recommended in the protocol?
You may prepare a standard range to suit your specific needs. However, the dynamic range has already been optimized, and…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, should I store the coated plates wet or dry?
You may store the coated plates for short periods of time (2-3 days) in blocking buffer, being careful to seal…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will my plate coating technique affect the precision of my plates and the overall assay?
It is possible for plate coating technique to lead to poor precision. Please refer to the insert for specific recommendations.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how can I get the greatest stability out of the coated plates?
After blocking, shake out all excess solution and store desiccated at 4°C.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how can I avoid evaporation of the solutions while coating, blocking, and assaying?
Seal the plates with a plate sealer, plastic wrap, or foil during incubations.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use a different buffer to dilute my standards?
Use of buffers other than those recommended will require validation by the user.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, what kind of substrate should I use to develop the plate?
Any colorimetric peroxidase substrate compatible with the enzyme of choice as well as your plate reader should be acceptable. See…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use a chemiluminescent substrate for more sensitivity?
Use of a chemiluminescent substrate is not validated and may require additional assay optimization.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023