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ELISA Immunoassays
Will the cytokine ELISA kits from one species work with another species?
This will depend on the species homology. Human cytokine ELISA kits will generally not measure cytokines in other species. Mouse…
Tue, Jul 18, 2023
What species can the eicosanoid ELISA kits be used for?
Eicosanoids are not species-specific. However, the antisera used in a particular assay may present an interference effect due to the…
Tue, Jul 18, 2023
When using an eicosanoid ELISA kit, how much indomethacin should I use in my samples?
You should follow the suggestion in the product specific manual, and add up to 10µg per ml of liquid sample.…
Tue, Jul 18, 2023
If I have 2N NaOH hydrolyzed samples for an 8-iso-PGF2a determination, do I need to extract these samples?
No, the Direct 8-iso-PGF2a kit is specifically designed to help you obtain results as quickly and easily as possible. The…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023
Can I measure urinary steroid levels in Enzo’s hormone ELISA kits?
Steroids are relatively insoluble and must be altered by being conjugated to glucoronic acid for elimination. The antibodies in our…
Tue, Jul 18, 2023
Do I need to add Steroid Displacement Reagent to my samples prior to analysis with my hormone ELISA kits?
If total steroid levels are to be measured, Steroid Displacement Reagent (SDR) should be added to the samples containing binding…
Tue, Jul 18, 2023
I am using the Direct 8-iso PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit. After I add the HCl to neutralize my samples, it is not reading in the range of pH 6-8. What should I do?
At this point, you have added HCl to NaOH which will yield NaCl + H2O. There is little buffering capacity…
Tue, Jul 18, 2023
Do eicosanoid assays require an extraction?
The majority of samples do not require an extraction. However, in some situations, an extraction will be required when the…
Tue, Jul 18, 2023
What antibody pairs are used in the kit?
This varies from kit to kit. Refer to the product specific instruction manual for more information.
Tue, Jul 18, 2023