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Detection Kits
Can you share the recipe of the Cell Growth Medium?
This information is considered to be proprietary and we cannot disclose the actual recipe. It contains additives and nutrients. These…
Tue, Jul 18, 2023
Why was Wnt3a chosen as agonist for the LEADING LIGHT® Wnt reporter cell line?
Wnt3a is the most commonly studied agonist of the canonical Wnt signaling pathway and that is why it was selected…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
How can I improve the intensity of the signal obtained with the LEADING LIGHT® Wnt reporter cells?
Conditions in terms of cell density, culture medium, and incubation have to be respected. It is very important that there…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay (ENZ-KIT130), what is the best incubation time, 4 hours or overnight?
An overnight incubation will give better results, especially with cells that may have lower SA-b-galactosidase activity.
Tue, Jul 18, 2023
Can your cellular senescence live cell analysis assay (ENZ-KIT130) be used with non-adherent cells?
ENZ-KIT130 can be used on any mammalian cells, including suspension cells. The only protocol modification required is to spin down…
Tue, Jul 18, 2023
Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023
How is your senescence live cell analysis assay any different from an X-Gal assay?
This assay detects the same activity as X-Gal but it is more sensitive. If a visual difference is detected with…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023
What is the detection mechanism used in your cellular senescence activity assay (ENZ-KIT129)?
Concerning the components of the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, the hypoxia detection reagent is relatively stable and can…
Tue, Jul 18, 2023