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Detection Kits
What information can you share about the LEADING LIGHT® Wnt reporter cell line?
The LEADING LIGHT® Wnt reporter cell line is a genetically engineered 3T3 mouse fibroblast cell line that contains a luciferase…
Tue, Jul 18, 2023
Can I use the LEADING LIGHT® Wnt reporter cells straight from the liquid nitrogen?
We would advise against using the cells from the liquid nitrogen. Cells need to be in their exponential growing phase…
Tue, Jul 18, 2023
Do I need to use the LEADING LIGHT® Wnt cell line medium pack?
The LEADING LIGHT® Wnt cell line medium pack (ENZ-60003) is made of a cell freezing medium, a cell growth medium…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay (ENZ-KIT130), what is the best incubation time, 4 hours or overnight?
An overnight incubation will give better results, especially with cells that may have lower SA-b-galactosidase activity.
Tue, Jul 18, 2023
How is your senescence live cell analysis assay any different from an X-Gal assay?
This assay detects the same activity as X-Gal but it is more sensitive. If a visual difference is detected with…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023
Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023
Can your cellular senescence live cell analysis assay (ENZ-KIT130) be used with non-adherent cells?
ENZ-KIT130 can be used on any mammalian cells, including suspension cells. The only protocol modification required is to spin down…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023