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Cellular Analysis
Why was Wnt3a chosen as agonist for the LEADING LIGHT® Wnt reporter cell line?
Wnt3a is the most commonly studied agonist of the canonical Wnt signaling pathway and that is why it was selected…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
I expect calcineurin to be mainly located in the nuclei. How should I treat my cell lysates in order to avoid loss of nuclei whe using Calcineurin cellular activity assay kit, BML-AK816?
You need to replace the high speed centrifugation suggested in the Calcineurin cellular activity assay kit (BML-AK816) manual which pellets…
Tue, Jul 18, 2023
Do light or divalent cations such as Ca2+ or Mg2+ interfere with NO measurement by DAF-2?
M. Broillet, et al (2001) and J. Chatton, et al (2002) reported that light and divalent cations, such as Ca2+…
Tue, Jul 18, 2023
Does DAF-2 react with ascorbic acid?
X. Zhang, et al. (2002) reported that DAF-2 reacted with dehydroascorbic acid (DHA) and ascorbic acid (AA) to generate fluorescent…
Tue, Jul 18, 2023
What is the recommend Calmodulin concentration to fully activate Calcinuerin in the Calcineurin Phosphatase assay kit, BML-AK804?
We provide 25µM Calmodulin with the Calcineurin Phosphatase assay kit (BML-AK804) and recommend a concentration of 0.5µM to fully activate…
Tue, Jul 18, 2023
Can the desalting resin provided with the kit BML-AK816, Calcineurin cellular activity assay kit be reused?
Yes, the desalting resin provided with the Calcineurin cellular activity assay kit (BML-AK816) can be reused for multiple samples. Between…
Tue, Jul 18, 2023
In the manual for the Calcineurin cellular activity assay kit, BML-AK816, you recommend using 5,000-50,000 cells per assay point. How much lysis buffer should be used to prepare the samples?
it is easier to dilute samples than to concentrate them. In order to prepare cells for use with Calcineurin cellular…
Tue, Jul 18, 2023
Could you recommend a control compound Calcineurin inhibitor to use with the Calcineurin Phosphatase assay kit, BML-AK804?
In the kit insert for our Calcineurin Phosphatase assay kit, BML-AK804, we list several inhibitors that were screened with the…
Tue, Jul 18, 2023
Can the kit BML-AK804, Calcineurin Phosphatase assay kit be used for biological samples?
The product BML-AK804, Calcineurin Phosphatase assay kit is designed for inhibitor screening or kinetic assays using the recombinant Calcineurin enzyme…
Tue, Jul 18, 2023