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Cellular Analysis
What are the advantages of the Cyto-ID® Autophagy Detection kit relative to GFP-LC3 transfected cells?
The most significant advantage for Cyto-ID® Autophagy Detection kit is that it's a no-transfection assay. Transfections are transient and there…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
What is the detailed nature of the Cyto-ID® autophagy dye and how does the assay work?
The precise chemical structure of the dye is proprietary. The probe is a cationic amphiphilic tracer (CAT) dye that rapidly…
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
Little or no apoptosis observed or detected
None or few apoptotic cells present in sample >> Use the Apoptosis Inducer, provided in the kit, as a positive…
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
What is the amount or concentration of SIRT1 provided in BML-AK555?
The volume or concentration of SIRT1 is lot-dependent. Each kit contains 100 units of the enzyme. Concentration on specific lot of SIRT1 is available…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023