Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Cellular Analysis
How stable is the Cyto-ID® Green autophagy dye?
Fluorescent dyes are subject to photobleaching, but this dye is more robust than dyes like fluorescein. Standard handling precautions should…
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
Which cell lines and primary cells/tissues have been tested with Cyto-ID® autophagy dye?
Some representative cells and cell lines tested with the kit include hepatocytes, SK-N-SH neuroblastoma cells, Chinese hamster ovary (CHO) cells,…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
What are the excitation and emission spectra of the FLUOR DE LYS® substrate?
The fluorophore is excited with 360nm light and the emitted light is detected at 460nm on a fluorometric microplate reader.…
Tue, Jul 18, 2023
Can you measure the activity of SIRT1 with the FLUOR DE LYS® HDAC fluorometric activity assay kit?
This kit measures the activity of all HDACs provided with a biological sample. Simply changing the substrate from the FLUOR…
Tue, Jul 18, 2023
What is the recommended substrate for the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit?
The FLUOR DE LYS® SIRT1 substrate, the recommended substrate, is provided in the kit. The FLUOR DE LYS® SIRT1 substrate is a…
Tue, Jul 18, 2023