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Cellular Analysis
Little or no apoptosis observed or detected
None or few apoptotic cells present in sample >> Use the Apoptosis Inducer, provided in the kit, as a positive…
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
What is the detailed nature of the Cyto-ID® autophagy dye and how does the assay work?
The precise chemical structure of the dye is proprietary. The probe is a cationic amphiphilic tracer (CAT) dye that rapidly…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
Can you measure the activity of SIRT1 with the FLUOR DE LYS® HDAC fluorometric activity assay kit?
This kit measures the activity of all HDACs provided with a biological sample. Simply changing the substrate from the FLUOR…
Tue, Jul 18, 2023
Can I have further details about the HeLa nuclear extract included in your HDAC activity assay kits?
The HeLa nuclear extract is prepared by high salt extraction of HeLa nuclei using a modified protocol from the ones…
Tue, Jul 18, 2023
Can I use the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit to measure SIRT1 activity in my cell/tissue extracts?
BML-AK555 is a drug discovery kit intended for screening potential activators or inhibitors of the recombinant SIRT1 provided in the…
Tue, Jul 18, 2023
What are the excitation and emission spectra of the FLUOR DE LYS® substrate?
The fluorophore is excited with 360nm light and the emitted light is detected at 460nm on a fluorometric microplate reader.…
Tue, Jul 18, 2023