Is there a requirement for cell lysis with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001
LEADING LIGHT® Wnt Reporter Assay (ENZ-61001) is a cell-based luciferase and does not require lysis and chemiluminescence signals can be…
Tue, Jul 18, 2023
What could be the reason for low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001?
If observing a low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay (ENZ-61001), please consider the following. The…
Tue, Jul 18, 2023
Is Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 compatible with anti-fluorescence quenching agent and fluoromount-G?
The CYTO-ID® Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 kits is compatible with anti-fluorescence quenching agent and Fluoromount-G.
Tue, Jul 18, 2023
Can the microplate be read following addition of ROS/Superoxide Detection Solution with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010?
Yes, plates can be read using bottom reading option without removing the ROS/Superoxide Detection Solution and without washing.
Tue, Jul 18, 2023
What volume of recombinant PKC or cell extracts should be used with ADI-EKS-420A?
In experiments with mouse brain lysates using the PKC kinase activity kit (ADI-EKS-420A), we found that 0.02 – 2µg of…
Tue, Jul 18, 2023
Which PKC isoform is supplied as the active enzyme in the PKC kinase activity kit, ADI-EKS-420A?
The PKC kinase activity kit (ADI-EKS-420A) contains recombinant PKCµ as positive control.
Tue, Jul 18, 2023
What is the difference between Calcineurin Phosphatase assay kit, BML-AK804 and Calcineurin cellular activity assay kit, BML-AK816?
Calcineurin Phosphatase assay kit (BML-AK804) is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. This kit comes with…
Tue, Jul 18, 2023
What is the difference between Hoechst 3342, ENZ-52401 and Hoechst 33258, ENZ-52402?
Hoechst 33258 (ENZ-52402) is less cell permeable than other derivatives, however it also allows for quantitative measurements when plotted in…
Tue, Jul 18, 2023
How does MITO-ID® dye in the Membrane Potential kits, ENZ-51018 and ENZ-51019 detect membrane potential?
MITO-ID® Membrane Potential Kits (ENZ-51018, ENZ-51019) measures mitochondrial membrane potential with a cationic dye dual-emission dye that exists as green…
Tue, Jul 18, 2023
What advantages does MITO-ID® Membrane Potential Kits, ENZ-51018 and ENZ-51019 offer compared to JC-1 and JC-10?
Compared with the commonly used cationic carbocyanine dye JC-1 and JC-10, the MITO-ID® membrane potential dye (ENZ-51018 and ENZ-51019) offers…
Tue, Jul 18, 2023
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