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Cellular Analysis
Is Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 compatible with anti-fluorescence quenching agent and fluoromount-G?
The CYTO-ID® Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 kits is compatible with anti-fluorescence quenching agent and Fluoromount-G.
Tue, Jul 18, 2023
Can the microplate be read following addition of ROS/Superoxide Detection Solution with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010?
Yes, plates can be read using bottom reading option without removing the ROS/Superoxide Detection Solution and without washing.
Tue, Jul 18, 2023
Is there a requirement for cell lysis with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001
LEADING LIGHT® Wnt Reporter Assay (ENZ-61001) is a cell-based luciferase and does not require lysis and chemiluminescence signals can be…
Tue, Jul 18, 2023
What could be the reason for low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001?
If observing a low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay (ENZ-61001), please consider the following. The…
Tue, Jul 18, 2023
What volume of recombinant PKC or cell extracts should be used with ADI-EKS-420A?
In experiments with mouse brain lysates using the PKC kinase activity kit (ADI-EKS-420A), we found that 0.02 – 2µg of…
Tue, Jul 18, 2023
What is the difference between Calcineurin Phosphatase assay kit, BML-AK804 and Calcineurin cellular activity assay kit, BML-AK816?
Calcineurin Phosphatase assay kit (BML-AK804) is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. This kit comes with…
Tue, Jul 18, 2023
What is the difference between Hoechst 3342, ENZ-52401 and Hoechst 33258, ENZ-52402?
Hoechst 33258 (ENZ-52402) is less cell permeable than other derivatives, however it also allows for quantitative measurements when plotted in…
Tue, Jul 18, 2023
Which PKC isoform is supplied as the active enzyme in the PKC kinase activity kit, ADI-EKS-420A?
The PKC kinase activity kit (ADI-EKS-420A) contains recombinant PKCµ as positive control.
Tue, Jul 18, 2023
How does MITO-ID® dye in the Membrane Potential kits, ENZ-51018 and ENZ-51019 detect membrane potential?
MITO-ID® Membrane Potential Kits (ENZ-51018, ENZ-51019) measures mitochondrial membrane potential with a cationic dye dual-emission dye that exists as green…
Tue, Jul 18, 2023
What advantages does MITO-ID® Membrane Potential Kits, ENZ-51018 and ENZ-51019 offer compared to JC-1 and JC-10?
Compared with the commonly used cationic carbocyanine dye JC-1 and JC-10, the MITO-ID® membrane potential dye (ENZ-51018 and ENZ-51019) offers…
Tue, Jul 18, 2023