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Cellular Analysis
High fluorescence signal observed in negative control
Sample contains interfering substances. The assay is compatible with commonly used buffers (PBS, Tris, HEPES) and excipients (trehalose and sucrose),…
Tue, Jul 18, 2023
More than one aggregation peak is observed.
A protein might have multiple domains that start aggregation at different temperatures. For most samples, any aggregation is an indication…
Tue, Jul 18, 2023
Inconsistent results between PDI assay experiments.
This is caused by inappropriate Stop Reagent addition. Be sure to pre-incubate with Stop Reagent to terminate both the enzyme…
Tue, Jul 18, 2023
Poor signal observed in the assay
Staining reagent has been exposed to strong light. Protect reagents from exposure to strong light.
Tue, Jul 18, 2023
High fluorescent signal at start of run
Protein formed aggregates before the run started. Use a different buffer or additive. Gain setting is too high. Many thermocyclers…
Tue, Jul 18, 2023
Insulin does not go into solution.
Insulin was not reconstituted in deionized water prior to dilution. Resuspend Insulin in deionized water before dilution into PBE Buffer.
Tue, Jul 18, 2023
What type of media is best for use in ADI-907-012 and ADI-907-015 H2O2 assays?
Media containing phenol red as a pH indicator will interfere in the assay. Most media are available with this removed…
Tue, Jul 18, 2023
Can whole blood samples be used?
High background may result from the presence of hemoglobin in your sample. Hemoglobin (Hb) has a significant absorption at 340…
Tue, Jul 18, 2023
My first assay for total glutathione worked well, but when I repeated the assay to test the oxidized samples, the curve was essentially flat-to-negative.
Oxidized glutathione is very difficult to detect because it is rapidly turned over and converted, so there is only a…
Tue, Jul 18, 2023
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023