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Cellular Analysis
What are the main advantages of working with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027?
The ProteoStat® Thermal Shift Stability Assay kit works over a wide temperature, pH and ionic strength range and is compatible…
Tue, Jul 18, 2023
What does ProteoStat® dye bind to and what is the mechanism of detection of protein aggregation?
The molecular rotor dye is non-fluorescent due to free rotation around a central carbon-carbon single bond separating different aromatic portions of…
Tue, Jul 18, 2023
Are there any limitations regarding particle size of the aggregates that can be detected with the ProteoStat® dye?
The ProteoStat® dye detects in the subvisible to visible range from ~ 50 µm to 1 mm.
Tue, Jul 18, 2023
Two peaks with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, were observed. What is the explanation?
One possible explanation for the two peaks is the presence of two proteins in the sample. If the test protein contains…
Tue, Jul 18, 2023
If the test protein is a variant with near identical amino acid sequence with the exception of one residue, is one peak expected ?
There may be a possibility of different conformations of the same protein aggregating at different temperatures. The sample could have…
Tue, Jul 18, 2023
What thermal cyclers have the protocol been tested with ProteoStat® Thermal shift stability assay kit, ENZ-51027?
ProteoStat® Thermal shift stability assay should work on all types of thermal cyclers in the market. It has been tested…
Tue, Jul 18, 2023
What detergents are compatible with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027 and ProteoStat® Protein Aggregation Assay?
The ProteoStat® dye is compatible with some detergents, such as 0.1% Tween 20, but this varies with the protein used. …
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023
Can the ProteoStat® TS Detection Reagent in the thermal shift stability assay kit, ENZ-51027, be substituted with the detection reagent from the ProteoStat® Protein Aggregation Assay, ENZ-51023?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
Would the aggregation temperature as determined using ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, be the same as determined using the Thermofluor technique?
In Thermofluor technique, SYPRO Orange, an environment-sensitive dye, is used to measure the unfolding of the protein as the temperature…
Tue, Jul 18, 2023