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Cellular Analysis
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
It appears that the positive control is not working properly. What could be the reason?
The nature of that protein is that it can quickly degrade unless it is handled very carefully. It must be…
Tue, Jul 18, 2023
What is the positive control provided in the kit?
The positive control provided in the kit ADI-EKS-390A is recombinant human PKAc beta (or catalytic subunit C-beta of PKA) expressed…
Tue, Jul 18, 2023
Can I use this kit to measure the concentration of PKA in my samples?
The kit does not measure the concentration of PKA in samples. It is intended for looking at relative PKA kinase activity.
Tue, Jul 18, 2023
What is the principle of the PKA kinase activity assay?
The PKA kinase activity kit allows relative quantification of PKA kinase activity in different sample types (purified, partially purified and…
Tue, Jul 18, 2023
Can I use my lysis buffer instead of the lysis buffer indicated in the kit insert?
The buffer suggested in the kit insert has been optimized by our development scientists. It is likely that an alternative buffer will work,…
Tue, Jul 18, 2023
Does the kit work for species other than human?
The ADI-EKS-390A kit is actually species independent; therefore, as long as there is sufficient PKA activity in your samples and…
Tue, Jul 18, 2023
Can the detection reagent from ProteoStat® Protein Aggregation Assay, ENZ-51023, be substituted with ProteoStat® TS Detection Reagent from the thermal shift stability assay kit, ENZ-51027?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
Would membrane proteins work with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat® Detection Reagent in ProteoStat® Protein Aggregation Assay, ENZ-51023?
We have not tested the ProteoStat® Protein Aggregation Assay, ENZ-51023, or ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, with membrane…
Tue, Jul 18, 2023