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Cellular Analysis
Does Cellular senescence activity assay, ENZ-KIT129 require running standards and are standards provided with this kit?
Cellular senescence activity assay, ENZ-KIT129 is designed as semi quantitative assay. Therefore, standards are not provided. As shown on page 7…
Tue, Jul 18, 2023
Are the reagents in MITO-ID® Membrane potential detection kit, ENZ-51018 sterile? Can the reagents in this kit be used in cell culture and flow cytometry application?
Reagents in MITO-ID® Membrane potential detection kit, ENZ-51018 are not sterile. The reagents in this kit are optimized for measurement of…
Tue, Jul 18, 2023
Why do we recommend 2,500 fold dilution of MITO-ID® Red Detection reagent, ENZ-51007 for staining aldehyde fixed and detergent permeabilized cells, but 10,000 fold dilution of MITO-ID® Detection reagent for live cells?
In aldehyde-fixed and detergent permeabilized sample, MITO-ID® Red Detection reagent diffuses passively once the membrane potential is lost. Therefore, higher dye…
Tue, Jul 18, 2023
Are results from Creatinine colorimetric detection kit, ADI-907-030A and Creatinine colorimetric detection kit, ADI-907-030 comparable?
Data obtained from Creatinine colorimetric detection kit, ADI-907-030A and Creatinine colorimetric detection kit, ADI-907-030 are comparable for the same set…
Tue, Jul 18, 2023
What is the recommended concentration of MG132, BML-PI102 used in proteasome research?
Recommend concentrations of MG132, BML-PI102 typically ranges from 10 uM to 150 uM and is cell dependent. We have not established…
Tue, Jul 18, 2023
In the reformulated BIOMOL® Green, BML-AK111, Tergitol15-S-9 replaces NP-40 detergent. While NP-40 is nonionic (no charge), would the switch cause any change in the overall properties or reaction rates?
Tergitol15-S-9 is also non-ionic in nature and this switch from a chemically similar detergent should not affect rates of reaction…
Tue, Jul 18, 2023
Would the results obtained with Creatinine colorimetric detection kit, ADI-907-030 and Creatinine colorimetric detection kit, ADI-907-030A be identical?
Results obtained with old and new version kit would not be identical. But, the values would be close and comparable…
Tue, Jul 18, 2023
What buffer should be used to dilute standards, ATP and active PKC in ADI-EKS-420A, PKC Kinase Activity Assay Kit?
Only Kinase Assay Dilution Buffer (80-1486) provided with PKC kinase activity kit (ADI-EKS-420A) should be used to dilute ATP, Standards…
Tue, Jul 18, 2023
Can test compound dissolved in DMSO be used in cell based assay to measure Reactive Oxygen Species with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010
We cannot confirm if dissolving the compound in DMSO could affect the assay, since DMSO could inhibit hydroxyl radical generation…
Tue, Jul 18, 2023
Can cells be fixed with 4% paraformaldehyde with the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175)?
A fixation step may be included when using the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175), but it needs to…
Tue, Jul 18, 2023