CELLESTIAL® Assays

Can different colored dyes be used to measure a different parameter with eThe eFluxx-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is possible to use a dye which would not have spectral overlap with the EFLUXX-ID® dyes (ENZ-51029, ENZ-51030) and…
Tue, Jul 18, 2023

Can DAPI be used as counterstaining for the nucleus with the nucleolar green detection kit Nucleolar-ID® Green detection kit for microscopy ENZ-51009 ?
Hoechst 33342 has been shown to work well. DAPI and Hoechst have similar spectral characteristics and either of them should work…
Tue, Jul 18, 2023

Can cells be fixed and permeabilized after staining with Nuclear-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006)?
Cells can be fixed and permeabilized after staining with the NUCLEAR-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006). For best results,…
Tue, Jul 18, 2023

What is the chemical identity of the LYSO-ID® dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
The precise chemical identity of the LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 is proprietary. These are a cell…
Tue, Jul 18, 2023

What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023

Antifade mounting medium hardset (ENZ-53002, ENZ-53003) appears to be clumped. What is the recommendation to get this in liquid form?
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) needs to be warmed at 37-42°C for about 20 minutes, then vortexedt, if clumps are…
Tue, Jul 18, 2023

Can antifade mounting medium hardset (ENZ-53002, ENZ-53003) be used with fluorescein and rhodamine?
Yes, the antifade mounting medium hardeset (ENZ-53002, ENZ-53003) is recommended for use on fiwed cells and tissus that have been…
Tue, Jul 18, 2023

What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?
The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…
Tue, Jul 18, 2023

Does the appearance of green aggregates under the microscope suggest increased mitochondrial potential with Mito-ID® Membrane potential detection kit, ENZ-51018?
The MITO-ID® dye in the MITO-ID® Membrane potential detection kit (ENZ-51018) is a dual-emission cationic dye to measure the mitochondrial…
Tue, Jul 18, 2023

How long does the signal last with Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019?
Time-course study of mitochondrial membrane potential changes with the Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019 can be…
Tue, Jul 18, 2023