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Home Life Sciences Life Sciences Products Cellular Analysis CELLESTIAL® Assays Page 3

CELLESTIAL® Assays

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Applied Sciences

Is it possible to multiplex MITO-ID® Membrane Potential Detection Kit, ENZ-51018 with NUCLEAR-ID® Blue/Red Cell Viability Reagent (GFP-CERTIFIED®), ENZ-53005?

MITO-ID/NUCLEAR-ID (ENZ-51018/ENZ-53005) multiplex assay have been multiplexed in a 3D spheroid cultures of iPSC-derived human hepatocytes (iCell Hepatocytes 2.0) assay…

Tue, Jul 18, 2023

Organelle Detection
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How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?

MITO-ID® Red detection (ENZ-51007)  and MITO-ID® Green Detection kit (ENZ-51002)  appear to target the inner mitochondrial membrane in mammalian cells, which…

Tue, Jul 18, 2023

Organelle Detection
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Can Polymount be used with a coverslip to image fixed cells stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035? Is the fluorescence stable to image following day?

Solvents should be avoided for imaging specimens stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035, as this is known to cause…

Tue, Jul 18, 2023

Aggregate Detection
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What is the mechanism of the Golgi-ID™ Green assay kit

The Green detection reagent included in the kit ENZ-51028 is a fluorescent sphingolipid analog that is metabolized by cells and…

Tue, Jul 18, 2023

Organelle Detection
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What is the difference between PROTEOSTAT® Aggresome detection kit (ENZ-51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023)?

Both our PROTEOSTAT® Aggresome detection kit (ENZ51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023) rely on the properties of our PROTEOSTAT®…

Tue, Jul 18, 2023

Aggregate Detection
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Do light or divalent cations such as Ca2+ or Mg2+ interfere with NO measurement by DAF-2?

M. Broillet, et al (2001) and J. Chatton, et al (2002) reported that light and divalent cations, such as Ca2+…

Tue, Jul 18, 2023

CELLESTIAL® Assays
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Does DAF-2 react with ascorbic acid?

X. Zhang, et al. (2002) reported that DAF-2 reacted with dehydroascorbic acid (DHA) and ascorbic acid (AA) to generate fluorescent…

Tue, Jul 18, 2023

CELLESTIAL® Assays
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What is the difference between storing a product at -70°C and -80°C?

Products with instructions to be stored at either of these temperatures can be stored at =-70°C. Storage at -70°C or…

Tue, Jul 18, 2023

Apoptosis/Necrosis Detection
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Is the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, specific for live cell imaging? Can cells be fixed after staining?

Yes, the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is validated on living cells only and tt would not be possible…

Tue, Jul 18, 2023

Oxidative Stress Detection
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Can ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, be used for microplate assay format?

ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, has not been validated in microplate format. The kit is validated for fluorescence microscopy and…

Tue, Jul 18, 2023

Oxidative Stress Detection
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