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CELLESTIAL® Assays
How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?
MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51002) appear to target the inner mitochondrial membrane in mammalian cells, which…
Tue, Jul 18, 2023
How can background staining be minimized with ORGANELLE-ID-RGB® III assay kit, ENZ-51032?
When using ORGANELLE-ID-RGB® III assay kit (ENZ-51032) the cell medium could be a problem. Staining can be improved by…
Tue, Jul 18, 2023
Can Polymount be used with a coverslip to image fixed cells stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035? Is the fluorescence stable to image following day?
Solvents should be avoided for imaging specimens stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035, as this is known to cause…
Tue, Jul 18, 2023
What is the difference between PROTEOSTAT® Aggresome detection kit (ENZ-51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023)?
Both our PROTEOSTAT® Aggresome detection kit (ENZ51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023) rely on the properties of our PROTEOSTAT®…
Tue, Jul 18, 2023
What is the mechanism of the Golgi-ID™ Green assay kit
The Green detection reagent included in the kit ENZ-51028 is a fluorescent sphingolipid analog that is metabolized by cells and…
Tue, Jul 18, 2023
Do light or divalent cations such as Ca2+ or Mg2+ interfere with NO measurement by DAF-2?
M. Broillet, et al (2001) and J. Chatton, et al (2002) reported that light and divalent cations, such as Ca2+…
Tue, Jul 18, 2023
What is the difference between storing a product at -70°C and -80°C?
Products with instructions to be stored at either of these temperatures can be stored at =-70°C. Storage at -70°C or…
Tue, Jul 18, 2023
Does DAF-2 react with ascorbic acid?
X. Zhang, et al. (2002) reported that DAF-2 reacted with dehydroascorbic acid (DHA) and ascorbic acid (AA) to generate fluorescent…
Tue, Jul 18, 2023
Under what conditions were the cells subjected to create hypoxic conditions with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042? Were the cells grown under 95% N2 5 % CO2 ?
When validating the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, cells were grown in 95% nitrogen and also validated with either…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023