CELLESTIAL® Assays

Can you provide an overview of steps in Intracellular Calcium mobilization assay with FLUOFORTE® Calcium assay kit, ENZ-51016 ?
The main steps for the FLUOFORTE® Calcium assay kit (ENZ51016) involves loading cells with dye that fluoresce when exposed to…
Tue, Jul 18, 2023

What precautions do we recommend while working with PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
The PROTEOSTAT® Detection Reagent in all these references (ENZ-51023, ENZ-51035 and ENZ-51027) is light sensitive. Avoid direct exposure of the…
Tue, Jul 18, 2023

How can photobleaching be minimized with PROTEOSTAT® Aggresome detection kit, ENZ-51035?
PROTEOSTAT® dye is light sensitive. Avoid direct exposure of the reagent to intense light. In a microscopy experiment with PROTEOSTAT®…
Tue, Jul 18, 2023

What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023

Why do we suggest 96 -well black wall microplate, preferably with a clear bottom for PROTEOSTAT® Protein aggregation assay, ENZ-51023
Black side wall microplates would minimize spillover of RFU reads between the neighboring wells when analyzing data obtained with PROTEOSTAT®…
Tue, Jul 18, 2023

How can staining be improved with the PROTEOSTAT® Aggresome detection kit, ENZ-51035?
Optimize the dilution of the dye provided with PROTEOSTAT® Aggresome detection kit (ENZ-51035) to improve staining. Also incubationb time can…
Tue, Jul 18, 2023

How does EFLUXX-ID® Green multidrug resistance assay kit, ENZ-51029 and EFLUXX-ID® Gold multidrug resistance assay kit, ENZ-51030 detect activity of transporter proteins?
EFLUXX-ID® Green and Gold Dyes in ENZ-51029 and ENZ-51030 kits are proprietary AM-esters. EFLUXX-ID® dye is a hydrophobic non-fluorescent compound…
Tue, Jul 18, 2023

How can background staining be minimized with ORGANELLE-ID-RGB® III assay kit, ENZ-51032?
When using ORGANELLE-ID-RGB® III assay kit (ENZ-51032) the cell medium could be a problem. Staining can be improved by…
Tue, Jul 18, 2023

What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?
It is important to check cell density and to ensure that cell concentration is 1 x 105 to 5 x…
Tue, Jul 18, 2023

Have any of Enzo Live Cell Fluorescent probes been validated on 3D spheroid culture?
While we did not specifically tested our Live Cell Analysis products on 3D models, we do have a few application…
Tue, Jul 18, 2023