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CELLESTIAL® Assays
High fluorescent signal at start of run
Protein formed aggregates before the run started. Use a different buffer or additive. Gain setting is too high. Many thermocyclers…
Tue, Jul 18, 2023
Inconsistent results between PDI assay experiments.
This is caused by inappropriate Stop Reagent addition. Be sure to pre-incubate with Stop Reagent to terminate both the enzyme…
Tue, Jul 18, 2023
Insulin does not go into solution.
Insulin was not reconstituted in deionized water prior to dilution. Resuspend Insulin in deionized water before dilution into PBE Buffer.
Tue, Jul 18, 2023
More than one aggregation peak is observed.
A protein might have multiple domains that start aggregation at different temperatures. For most samples, any aggregation is an indication…
Tue, Jul 18, 2023
The dyes fail to stain acidic organelles in fixed and/or permeabilized cells.
The following kits are only suitable for live cell staining only. Use them only for live cell analysis. Cyto-ID® Autophagy…
Tue, Jul 18, 2023
Poor staining is observed in the positive control.
Stained reagents have been exposed to strong light. Protect samples from exposure to strong light and analyze them immediately after…
Tue, Jul 18, 2023
Protein signal is saturated.
The concentration from protein sample is too high. Dilute the sample further with 1X Assay Buffer.
Tue, Jul 18, 2023
Nuclear counterstain is too bright compared to the organelle stain.
Different microscopes, cameras and filters may make some signals appear very bright. Reduce the concentration of the nuclear counterstain or…
Tue, Jul 18, 2023
Organelles are not sufficiently stained.
Very low concentration of dye was used, or dye was incubated with the cells for an insufficient length of time.…
Tue, Jul 18, 2023
Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023