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CELLESTIAL® Assays
Poor signal observed in the assay
Staining reagent has been exposed to strong light. Protect reagents from exposure to strong light.
Tue, Jul 18, 2023
More than one aggregation peak is observed.
A protein might have multiple domains that start aggregation at different temperatures. For most samples, any aggregation is an indication…
Tue, Jul 18, 2023
Inconsistent results between PDI assay experiments.
This is caused by inappropriate Stop Reagent addition. Be sure to pre-incubate with Stop Reagent to terminate both the enzyme…
Tue, Jul 18, 2023
Low or no fluorescent signal in positive control
Species of interest may react with each other, thus attenuating the expected signal. ROS is a complex interacting system. Blocking…
Tue, Jul 18, 2023
The dyes fail to stain acidic organelles in fixed and/or permeabilized cells.
The following kits are only suitable for live cell staining only. Use them only for live cell analysis. Cyto-ID® Autophagy…
Tue, Jul 18, 2023
Protein signal is saturated.
The concentration from protein sample is too high. Dilute the sample further with 1X Assay Buffer.
Tue, Jul 18, 2023
Nuclear counterstain is too bright compared to the organelle stain.
Different microscopes, cameras and filters may make some signals appear very bright. Reduce the concentration of the nuclear counterstain or…
Tue, Jul 18, 2023
Organelles are not sufficiently stained.
Very low concentration of dye was used, or dye was incubated with the cells for an insufficient length of time.…
Tue, Jul 18, 2023
Poor staining is observed in the positive control.
Stained reagents have been exposed to strong light. Protect samples from exposure to strong light and analyze them immediately after…
Tue, Jul 18, 2023
My cell culture samples are in DMEM. Will this interfere with the Nitric Oxide assay?
Yes. The nitrate salt used in this particular media will cause interference. It is important to check the published media…
Tue, Jul 18, 2023