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CELLESTIAL® Assays
Well-to-well variation observed
Incorrect dispensing and/or experimental setup parameter used. A fluorescence microplate reader with automatic dispensing should be used for assays requiring precise…
Tue, Jul 18, 2023
More than two populations appear upon apoptosis induction.
It's the property of the dye. Apoptosis is a multi-stage process and Nuclear-ID green dye can distinguish different levels of…
Tue, Jul 18, 2023
Healthy cells show very high fluorescence intensity
PMT setting is too high. Lower PMT setting on flow cytometer. Incubation temperature. Incubation with the dye should be at…
Tue, Jul 18, 2023
Poor Mito-ID staining observed.
Stained cells may have been exposed to strong light. Mito-ID® MP dye is light sensitive; be sure to protect samples…
Tue, Jul 18, 2023
In the sPLA2 activity kit (ADI-907-002), how is a Unit defined?
One unit of phospholipase activity is the amount of enzyme needed to hydrolyze 1.0 µmole of L-a-phosphatidylcholine to L-a-lysophosphatidylcholine and…
Tue, Jul 18, 2023
Poor signal observed in the assay
Staining reagent has been exposed to strong light. Protect reagents from exposure to strong light.
Tue, Jul 18, 2023
High fluorescent signal at start of run
Protein formed aggregates before the run started. Use a different buffer or additive. Gain setting is too high. Many thermocyclers…
Tue, Jul 18, 2023
DNA histograms are of poor overall quality.
The key elements in obtaining a histogram of high quality are good sample preparation, correct cell-to dye ratio and proper…
Tue, Jul 18, 2023
No decrease of the fluorescent signal after using a specific inhibitor
Inhibitor concentration is too low or too high. Very low doses of inhibitor may not affect ROS production by inducer.…
Tue, Jul 18, 2023
Low or no fluorescent signal in positive control
Species of interest may react with each other, thus attenuating the expected signal. ROS is a complex interacting system. Blocking…
Tue, Jul 18, 2023