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Aggregate Detection
What precautions do we recommend while working with PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
The PROTEOSTAT® Detection Reagent in all these references (ENZ-51023, ENZ-51035 and ENZ-51027) is light sensitive. Avoid direct exposure of the…
Tue, Jul 18, 2023
How can photobleaching be minimized with PROTEOSTAT® Aggresome detection kit, ENZ-51035?
PROTEOSTAT® dye is light sensitive. Avoid direct exposure of the reagent to intense light. In a microscopy experiment with PROTEOSTAT®…
Tue, Jul 18, 2023
What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023
Why do we suggest 96 -well black wall microplate, preferably with a clear bottom for PROTEOSTAT® Protein aggregation assay, ENZ-51023
Black side wall microplates would minimize spillover of RFU reads between the neighboring wells when analyzing data obtained with PROTEOSTAT®…
Tue, Jul 18, 2023
How can staining be improved with the PROTEOSTAT® Aggresome detection kit, ENZ-51035?
Optimize the dilution of the dye provided with PROTEOSTAT® Aggresome detection kit (ENZ-51035) to improve staining. Also incubationb time can…
Tue, Jul 18, 2023
Can Polymount be used with a coverslip to image fixed cells stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035? Is the fluorescence stable to image following day?
Solvents should be avoided for imaging specimens stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035, as this is known to cause…
Tue, Jul 18, 2023
What is the difference between PROTEOSTAT® Aggresome detection kit (ENZ-51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023)?
Both our PROTEOSTAT® Aggresome detection kit (ENZ51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023) rely on the properties of our PROTEOSTAT®…
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with gram negative bacteria?
LPS in outer membrane of gram negative bacteria can be problematic. The permeabilizing buffer described in the manual, would remove…
Tue, Jul 18, 2023