Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Competitive ELISA Assay
For small molecule analytes, typically less than 10 kDa, the competitive ELISA Kit is used. An advantage of this assay…
Tue, Jul 18, 2023
Checking for Interference or Linearity
Interference is when some component of a sample prevents the appropriate binding events to take place. Percent recovery is the…
Tue, Jul 18, 2023
Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023
Immunohistochemistry Protocol
Protocols for immunohistochemistry vary widely due to differences in antigens and their recognition by antibodies. Various fixation methods can uncover…
Tue, Jul 18, 2023
Applications
Abbreviation Full Name AA Activity Assay AC Affinity Chromatography CIE Crossed Immune Electrophoresis DB Dot Blot…
Tue, Jul 18, 2023
Non-Antibody Based Assays
Activity Assays Activity assays do not use antibodies as detection reagents. The kits measure the activity of enzymes by measuring…
Tue, Jul 18, 2023
Fluorescence Polarization Immunoassays
Fluorescence polarization immunoassays (FPIA) are quick and efficient homogeneous mix-and-read assays. The principle of the FPIA is similar to that…
Tue, Jul 18, 2023
Antibody Conjugation
Abbreviation Full Name PE R-Phycoerythrin DY DYTM is a trademark of the Dyomics , Inc. DyLightTM 488 …
Tue, Jul 18, 2023
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