
Immunoprecipitation of Phosphoserine-, Phosphothreonine- and Phosphotyrosine-Containing Proteins
Important Remarks: Rinse all plastic vessels with BSA solution (10 mg/mL in lysis buffer). Use lipid-free BSA, e.g., Sigma A7906. For…
Tue, Jul 18, 2023

Cell ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note:The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not been…
Tue, Jul 18, 2023

Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023

Paraformaldehyde/Glutaraldehyde Fixation
Paraformaldehyde/glutaraldehyde fixation(method of choice for double labeling of membrane bound and cytoskeletal antigens) Rinse cells with PBS at room temperature.…
Tue, Jul 18, 2023

Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023

Immunohistochemistry Protocol
Protocols for immunohistochemistry vary widely due to differences in antigens and their recognition by antibodies. Various fixation methods can uncover…
Tue, Jul 18, 2023

Applications
Abbreviation Full Name AA Activity Assay AC Affinity Chromatography CIE Crossed Immune Electrophoresis DB Dot Blot…
Tue, Jul 18, 2023

Non-Antibody Based Assays
Activity Assays Activity assays do not use antibodies as detection reagents. The kits measure the activity of enzymes by measuring…
Tue, Jul 18, 2023

Fluorescence Polarization Immunoassays
Fluorescence polarization immunoassays (FPIA) are quick and efficient homogeneous mix-and-read assays. The principle of the FPIA is similar to that…
Tue, Jul 18, 2023

Indirect Immunoassays
< The enzyme-linked immunosorbent assay (ELISA), also known as an indirect immunoassay, is a third type of immunoassay commonly used…
Tue, Jul 18, 2023