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If the test protein is a variant with near identical amino acid sequence with the exception of one residue, is one peak expected ?
There may be a possibility of different conformations of the same protein aggregating at different temperatures. The sample could have…
Tue, Jul 18, 2023
What thermal cyclers have the protocol been tested with ProteoStat® Thermal shift stability assay kit, ENZ-51027?
ProteoStat® Thermal shift stability assay should work on all types of thermal cyclers in the market. It has been tested…
Tue, Jul 18, 2023
What detergents are compatible with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027 and ProteoStat® Protein Aggregation Assay?
The ProteoStat® dye is compatible with some detergents, such as 0.1% Tween 20, but this varies with the protein used. …
Tue, Jul 18, 2023
How does the steroid displacement reagent (SDR) work?
Steroids in circulation are almost all ionically bound to carrier proteins such as albumin or more specific binding proteins. Treating…
Tue, Jul 18, 2023
Why is there sample dilution necessary in this ELISA Kit?
There are several reasons for dilutions. In some cases samples might read above the standard curve, thus requiring a dilution…
Tue, Jul 18, 2023
Which is the best dilution for my sample type?
In the manual, section “Sample recoveries”, we provide a list of recommended dilutions. Those are the dilutions our development scientists…
Tue, Jul 18, 2023
How can I overcome sample matrix interference?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine…
Tue, Jul 18, 2023
Do you sell positive controls that can be run with your steroid ELISA kits?
We do not provide positive control samples with our kits. If you need to ensure accurate recovery of any analyte…
Tue, Jul 18, 2023
What are the standard QC parameters this kit must accommodate?
For a typical labeling reaction with an input of 500 ng of high quality genomic DNA, the yield should be…
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023