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What is the minimum sample volume for using the kit?
The assay requires 100 µL of diluted sample per well. The amount needed, therefore, depends on the samples themselves and…
Tue, Jul 18, 2023
Do you check for cross-reactivity with other steroids?
Yes, cross-reactivities for a number of related steroid compounds were determined and are listed in the kit insert.
Tue, Jul 18, 2023
Can the detection reagent from ProteoStat® Protein Aggregation Assay, ENZ-51023, be substituted with ProteoStat® TS Detection Reagent from the thermal shift stability assay kit, ENZ-51027?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
Can the Cortisol ELISA kit be used with samples from any species?
Cortisol is conserved across all species and the antibody provided in the kit will bind to cortisol independently from the…
Tue, Jul 18, 2023
Would membrane proteins work with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat® Detection Reagent in ProteoStat® Protein Aggregation Assay, ENZ-51023?
We have not tested the ProteoStat® Protein Aggregation Assay, ENZ-51023, or ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, with membrane…
Tue, Jul 18, 2023
What are the main advantages of working with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027?
The ProteoStat® Thermal Shift Stability Assay kit works over a wide temperature, pH and ionic strength range and is compatible…
Tue, Jul 18, 2023
What does ProteoStat® dye bind to and what is the mechanism of detection of protein aggregation?
The molecular rotor dye is non-fluorescent due to free rotation around a central carbon-carbon single bond separating different aromatic portions of…
Tue, Jul 18, 2023
Two peaks with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, were observed. What is the explanation?
One possible explanation for the two peaks is the presence of two proteins in the sample. If the test protein contains…
Tue, Jul 18, 2023
If the test protein is a variant with near identical amino acid sequence with the exception of one residue, is one peak expected ?
There may be a possibility of different conformations of the same protein aggregating at different temperatures. The sample could have…
Tue, Jul 18, 2023
What thermal cyclers have the protocol been tested with ProteoStat® Thermal shift stability assay kit, ENZ-51027?
ProteoStat® Thermal shift stability assay should work on all types of thermal cyclers in the market. It has been tested…
Tue, Jul 18, 2023