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Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?
No, these are two different dyes.
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
Do you have any literature references for normal human 8-OHdG levels using ADI-EKS-350?
Unfortunately, there is no real “gold standard” for comparing normal or expected levels of 8-OHdG. The latter is a rather…
Tue, Jul 18, 2023
What do I need to do if my antibody concentration is higher than the 50 µg/mL indicated in the manual for ADI-900-057?
If you have samples with an antibody concentration higher than 50 µg/mL, you just need to dilute these samples down to…
Tue, Jul 18, 2023
Can I measure the levels of 8-OHdG in cell or tissue extracts?
The order of events happening in the cell is that the damaged DNA is modified, halting further transcription. Repair of…
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
Can cell lysates be used to determine protein aggregation with ProteoStat® Aggresome detection reagent?
Working with cell lysates would be challenging, because detergents can cause high background. It is possible to titrate the amount…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with prokaryote samples?
The dye should work with prokaryotes if membrane fractions are separated. If the membrane fraction is not removed, the dye…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with gram negative bacteria?
LPS in outer membrane of gram negative bacteria can be problematic. The permeabilizing buffer described in the manual, would remove…
Tue, Jul 18, 2023