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Knowledge Base
Can components of Enzo’s CGH labeling kits be purchased separately?
Unfortunately, we will not provide components of our CGH labeling kits as our kits are readily packaged and QCed together.…
Tue, Jul 18, 2023
When I opened the parcel containing the CGH labeling kit from Enzo, all of the dry ice had disappeared, will it still be good to use?
If the vials in the kit were still frozen when you received them then the product is okay to use.…
Tue, Jul 18, 2023
In which cases do you recommend to digest the DNA prior to labeling with Enzo’s CGH labeling kits?
For CGH+SNP arrays, genomic DNA must be digested using a combination of the restriction enzymes AluI and RsaI. For CGH…
Tue, Jul 18, 2023
What will be the effect of decreasing the labeling time with Enzo’s CGH labeling kits below the recommended 4 hours?
For input DNA of good quality and quantity, shorter labeling times down to 2 hours may be sufficient, but the…
Tue, Jul 18, 2023
What is the optimal time for hybridization of DNA that was labeled with Enzo’s CGH labeling kits?
Please follow the recommendations of the CGH array manufacturer you are using.
Tue, Jul 18, 2023
How should the Cyanine dyes of Enzo’s CGH labeling kits be stored after thawing?
For up to ten reactions, they can directly be refrozen at -20°C in the dark. For more reactions, they should…
Tue, Jul 18, 2023
Do I really need to add Stop Buffer after the labeling reaction with Enzo’s CGH labeling kits even though the enzyme is heat-inactivated?
During kit validation, a protocol utilizing a 4-hour labeling step and addition of Stop Buffer gave us the best results…
Tue, Jul 18, 2023
For the Protein A ELISA kit, ADI-900-057, would the readings be affected if the wells were to dry out when residual wash buffer is blotted out?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), care should be taken not to allow the…
Tue, Jul 18, 2023
For the Protein A ELISA kit (ADI-900-057) would residual wash buffer affect the assay?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), residual wash buffer can interfere with the assay…
Tue, Jul 18, 2023
Does the Protein A ELISA kit recognize denatured and non-denatured protein A?
A variety of Protein A constructs have been tested in our Protein A ELISA kit. Please refer to the product…
Tue, Jul 18, 2023