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What information can you share about the LEADING LIGHT® Wnt reporter cell line?
The LEADING LIGHT® Wnt reporter cell line is a genetically engineered 3T3 mouse fibroblast cell line that contains a luciferase…
Tue, Jul 18, 2023
Can you share the recipe of the Cell Growth Medium?
This information is considered to be proprietary and we cannot disclose the actual recipe. It contains additives and nutrients. These…
Tue, Jul 18, 2023
Why was Wnt3a chosen as agonist for the LEADING LIGHT® Wnt reporter cell line?
Wnt3a is the most commonly studied agonist of the canonical Wnt signaling pathway and that is why it was selected…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
How can I improve the intensity of the signal obtained with the LEADING LIGHT® Wnt reporter cells?
Conditions in terms of cell density, culture medium, and incubation have to be respected. It is very important that there…
Tue, Jul 18, 2023
Is the CYTAG™ CGH Labeling kit also suitable for BAC arrays?
The CYTAG™ CGH Labeling kit was designed for the more demanding high density oligo arrays, but it also works well…
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be handled?
We recommend aliquoting the kit reagents upon first use into volumes convenient for your workflow, and to avoid repeated freezing…
Tue, Jul 18, 2023
What are the key advantages of Enzo’s CGH labeling kits in comparison to the kits Agilent offers?
All our CGH labeling kits outperform the kits offered by Agilent for labeling: They provide excellent DNA yields with superior…
Tue, Jul 18, 2023
What are the molar extinction coefficients of the Cyanine 3 and Cyanine 5 dyes in Enzo’s CGH labeling kits?
Cyanine 3: 150,000 M cm-1; Cyanine 5: 250,000 M cm-1
Tue, Jul 18, 2023
Is there a way to quickly check for the labeling efficiency of Enzo’s CGH labeling kits?
Visual inspection after combining the Cyanine 3-labeled and Cyanine 5-labeled and cleaned DNA should be visibly purple. More detailed analysis…
Tue, Jul 18, 2023