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RNA Probe Design and Generation – How are RNA probes prepared?
RNA probes are usually prepared with biotin or specific fluorophore by in vitro transcription. High specific activity RNA probes or riboprobes…
Tue, Jul 18, 2023
How big are ISH probes?
There is no defined answer. Depending on the tissue specimen, target type, and sequence, ISH probes might vary from below…
Tue, Jul 18, 2023
Why are peptidases/proteinases used in ISH?
Peptidases or proteinases like proteinase K, pepsin or trypsin are used in ISH to digest proteins contained in the sample…
Tue, Jul 18, 2023
Is protein blocking really necessary in ISH?
Blocking with bovine serum albumin (BSA), milk or serum is always recommended when ISH signals are detected by antibodies (e.g.…
Tue, Jul 18, 2023
Why are the Gold 525 dUTPs bleeding through in the red channel?
If an epifluorescence microscope is used for visualization, the incorporated dUTPs should be matched to the filters equipped in the…
Tue, Jul 18, 2023
How can slides be stored after ISH?
That depends mostly on fixation/embedding method and the type of ISH performed. After chromogenic ISH on FFPE or cryopreserved tissue,…
Tue, Jul 18, 2023
Probe Design and Generation – How are DNA probes prepared?
First choice is to explore if commercially ready-to-use probes are available for specific targets. Enzo Life Sciences offers a selection…
Tue, Jul 18, 2023
Are the controls in AMPIPROBE® NG/CT/TV Extraction Controls, ENZ-GEN208-0500 quantitative or qualitative?
AMPIPROBE® NG/CT/TV Extraction Controls (ENZ-GEN208) are only qualitative and designed te used during genomic DNA extraction and is the qPCR…
Tue, Jul 18, 2023
How can RNA degradation in the sample be prevented?
When RNA is the target of ISH, preservation of RNA in the sample is very important given the ubiquitous presence…
Tue, Jul 18, 2023
Is there a specifc type of glass slide recommended for ISH?
Positively charged glass slides have proven optimal to draw tissue sample sections for subsequent ISH on. Alternatively, poly-L-lysine or silanized…
Tue, Jul 18, 2023