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Knowledge Base
What is the recommendation to reconstitute the SEEBRIGHT® lyophilized dUTPs, ENZ-42853L, ENZ-42843L, ENZ-42831L, ENZ-42842L?
Dissolving in 10 mM TE pH8, is the preferred method since the nucleotides are stable for long term -20°C storage.…
Tue, Jul 18, 2023
Do we offer a kit to make cDNA from a low amount of RNA in the range 10-100 ng sample?
For cDNA preparation from low input RNA samples, we offer AMPIGENE® cDNA Synthesis Kit, ENZ-KIT106. The AMPIGENE® cDNA Synthesis Kit uses the…
Tue, Jul 18, 2023
What information does Lot specific Certificate of Analysis for Protein A ELIA kit, ADI-900-057 have?
Lot specific Certificate of Analysis for Protein A ELISA kit (ADI-900-057) lists: assay range, OD values for High, Medium, Low,…
Tue, Jul 18, 2023
Can Sample diluent (80-1504) be used to dilute antibody if one runs out of antibody diluent (80-1759) with HO-1 (human), ELISA kit, ADI-EKS-800?
We do not recommend using Sample diluent (80-1504) to dilute antibody instead of antibody diluent (80-1759) with HO-1 (human), ELISA…
Tue, Jul 18, 2023
How many filters does the Absorbance 96 plate reader come with?
The Absorbance 96 plate reader comes equipped with four (4) filters with wavelengths of your choice.
Tue, Jul 18, 2023
Which wavelengths can the Absorbance 96 plate reader be equipped with?
Our standard device setup includes the following four (4) wavelengths: 405 nm, 450 nm, 492 nm, and 620 nm. Furthermore,…
Tue, Jul 18, 2023
Do custom wavelengths for the Absorbance 96 plate reader cost extra?
No, commonly used wavelength filters can be chosen at no extra cost. The vast majority of filter wavelengths and applications…
Tue, Jul 18, 2023
Can I order extra filters for the Absorbance 96 plate reader?
No, you cannot unfortunately. The Absorbance 96 plate reader comes with four (4) fixed installed filters and does not have…
Tue, Jul 18, 2023
Can Excel be used to plot read outs from ELISA? How to calculate reads from ELISA using Excel?
Our recommendation is to analyze ELISA reads by 4P curve fit. Many plate readers do have the ability to perform…
Tue, Jul 18, 2023
Why is 4P curve fit curve recommended for ELISA data analysis and where can I find access to this data analysis software?
For the most accurate results, we normally recommend to use a 4-parameter logistic (4-PL) curve fitting software, as 4-PL fitting…
Tue, Jul 18, 2023