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Flow Cytometry
Validated Antibodies, Assays and Reagents
Tools for Flow Cytometry
Flow cytometry is a laser-based technology used in cell sorting and analysis. Flow cytometry measures a particle’s size, granularity, and fluorescence intensity, and continues to a be a very useful tool. Its importance in scientific research and diagnosis continues increasing.
Flow cytometry allows researchers to measure several physical characteristics of cells including its shape, size, and internal complexity. It has long been used in cell counting and cell sorting< but more recently, researchers have been using flow cytometry in a broad range of applications including: biomarker detection, cell signaling, pathway screening, protein engineering, and systems biology. Flow cytometry is inherently a robust technique that adapts easily to basic research, drug development, and diagnostics.
Enzo offers a variety of flow cytometry-based assays for cell viability, proliferation, and death analysis, available in a wide range of formats to meet individual research needs. These flexible assays are compatible with all flow cytometry platforms, regardless of size or functionality. The assays are easy to perform and can be run quickly using small sample input, without sacrificing sensitivity. This portfolio has been designed to include multiple applications-driven configurations providing information on different parameters of interest.
A simple assay to quantify senescence-associated β-galactosidase activity in live cells using flow cytometry or fluorescent microscopy.
A no-transfection, quantitative live cell assay that identifies autophagy regulators by imaging and flow cytometry.
The only assay that detects aggresomes and aggresome-like inclusion bodies by flow cytometry.
Simple mix-and-read assay for detection of DNA content in live cells. Available with NUCLEAR-ID® Green Cell Cycle reagent or Red Cell Cycle reagent.
Non-toxic, fluorescent labeling of live cells that can be used over extended time periods. Available with CYTO-ID® Green Tracer dye or Red Tracer dye.
A live cell analysis assay for detection of nuclear condensation, one of the prominent hallmarks of apoptosis. Does not require installation of a specialized violet laser.
Enzo cell-based phenotypic assays directly monitor global levels of reactive oxygen species (ROS) and evaluate the functional status of mitochondrial membrane potential in live cells. These flexible assays are compatible with all flow cytometry platforms regardless of size or functionality. The assays are easy to perform and can be run quickly while providing robust signal and high sensitivity. This portfolio has been optimized to provide maximum information from a single test. Many of the kits are provided in multiplexed configurations.
Multiplex, Real-time Analysis of Hypoxia and ROS
Detect Activity of All Three Major ABC Transporter Proteins
The Enzo multidrug resistance assays are the only available kits for simultaneous monitoring of all three major ABC transporter proteins — MDR, MRP & BCRP. The high sensitivity of the assay delivers fewer false negative results, and provides data for BCRP activity not detected in Calcein AM assays. The kits are easily incorporated into a high content screening environment making them ideal for drug screening applications.
The Enzo’s portfolio of over 3,000 antibodies for cellular analysis includes more than 640 antibodies specifically validated for use in flow cytometry. These include isotype controls as well as biotin and fluorescent conjugates for use in evaluating intracellular and extracellular proteins, including:
- CD antigens
- Epitope tags
- Heat shock proteins (HSPs)
- Heme oxygenases
- TNF/TRAIL receptors
- Toll-like receptors (TLRs)
Flow Cytometry Antibody Search
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