MRP5 (human) monoclonal antibody (M5I-1)

Multidrug resistance-associated protein 5, ABCC5

Flow Cytometry, ICC, IHC (FS), WB

Flow Cytometry: weak, cell permeabilization required
Immunocytochemistry: cytospin preparations

M5I-1

Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP3.

Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.

Rat

Recombinant human MRP5 (multidrug resistance-associated protein 5) (aa 82-168).

IgG2a

Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.Immunohistochemistry (frozen sections, weak): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-rat Ig and a monoclonal rat APAAP complex. Alternatively, after incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat Ig and streptavidin conjugated to horseradish peroxidase.Western Blot: Use 1:20-1:50 dilution and anti-rat-HRP.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Human

Recognizes an internal epitope of human MRP5.

O15440

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