KillerTRAIL™ Protein (soluble) (human), (recombinant) (BULK)

ALX-201-123

8 citations

Datasheet
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Product Details

Alternative Name	Apo-2L, TNFSF 10, CD253
Biological Activity	Induces apoptosis in a concentration range of 10-100ng/ml. Note: This protein does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SUPERKILLERTRAIL^® (Prod. No. ALX-201-115).
Endotoxin Content	<10EU/µg protein (LAL test; Bio Whittaker).
Formulation	Liquid. In 20mM HEPES, pH 7.4, containing 150mM sodium chloride, 0.005% Tween 20, 1% sucrose, 1mM DTT.
Purity	≥98% (SDS-PAGE)
Purity Detail	Affinity purified.
Reconstitution	For a special storage and dilution buffer see KillerTRAIL™ Storage and Dilution Buffer (Prod. No. ALX-505-005).
Source	Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.
Specific Activity	ED₅₀: 20ng/ml (BJAB cells)
Specificity	Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG).
UniProt ID	P50591

Handling & Storage

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Exploiting the immunogenic potential of standard of care radiation or cisplatin therapy in preclinical models of HPV-associated malignancies: J.T. Kowalczyk, et al.; J. Immunother. Cancer 11, e005752 (2022), Abstract
Differences and similarities in TRAIL-and tumor necrosis factor-mediated necroptotic signaling in cancer cells: J. Sosna, et al.; Mol. Cell. Biol. 36, 2626 (2016), Abstract
The SMAC mimetic BV6 induces cell death and sensitizes different cell lines to TNF-α and TRAIL-induced apoptosis: M. El-Mesery, et al.; Exp. Biol. Med. 241, 2015 (2016), Abstract
Chronic lymphocytic leukemic cells exhibit apoptotic signaling via TRAIL-R1: M. MacFarlane, et al.; Cell Death Differ. 12, 773 (2005), Abstract
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors: M. Plasilova, et al.; Leukemia 16, 67 (2002), Abstract
TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Sträter, et al.; Gastroenterology 122, 659 (2002), Abstract
The cytokines tumor necrosis factor-alpha (TNF-alpha ) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papillomavirus-16 E7 oncoprotein: J.R. Basile, et al.; J. Biol. Chem. 276, 22522 (2001), Abstract — Full Text
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett. 503, 151 (2001), Abstract

Related Products

Available

SUPERKILLERTRAIL® Protein (soluble) (human), (recombinant)

ALX-201-115

Enhanced ligand with improved stability providing significantly enhanced immune activation.

37 citations

Alternative Name	TNFSF 10, Apo-2L, CD253
Purity	≥98% (SDS-PAGE). MS analysis, <1% impurity (mainly Hsp70 protein from E. coli)
Source	Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

ALX-201-115-C010 — 10 µg Available

$607.00

SUPERKILLERTRAIL® Protein (soluble) (human), (recombinant) quantity
ALX-201-115-3010 — 3×10 µg Available

$1,290.00

SUPERKILLERTRAIL® Protein (soluble) (human), (recombinant) quantity